Collybistin/ARHGEF9 Antibody (809309) [Unconjugated]

Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line, human brain (cortex) tissue, and mouse brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Collybistin/ARHGEF9 Monoclonal Antibody (Catalog # MAB7848) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Collybistin/ARHGEF9 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Collybistin/ARHGEF9 was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human Collybistin/ARHGEF9 Monoclonal Antibody (Catalog # MAB7848) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 809309
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Collybistin/ARHGEF9 Antibody (809309) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Collybistin/ARHGEF9
  Asn390-Lys516
  Accession # O43307
• Specificity: Detects human Collybistin/ARHGEF9 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ARHGEF9
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Collybistin/ARHGEF9 Antibody (809309) [Unconjugated]

• ARHGEF9
• Cdc42 guanine nucleotide exchange factor (GEF) 9
• Collybistin
• EIEE8
• hPEM-2 collybistin
• KIAA0424
• KIAA0424HPEM-2
• PEM-2 homolog
• PEM2
• PEM-2
• Rac/Cdc42 guanine nucleotide exchange factor 9
• rho guanine nucleotide exchange factor 9

Background

Collybistin, also known as ARHGEF9, is an approximately 60 kDa cytoplasmic guanine nucleotide exchange factor. It is expressed in the brain and is upregulated at the onset of neuronal differentiation. Collybistin associates with Neuroligin-2, Gephyrin, and GABA receptor subunits to form a complex which is required for GABAergic synaptogenesis as well as hippocampal synaptic plasticity. Mutations in Collybistin are associated with a variety of neurological disorders including epilepsy, anxiety, aggression, mental retardation, and hyperplexia. Human Collybistin contains an SH3 domain (aa 8-67), a DH domain (aa 103-287), and a Pleckstrin homology domain (aa 318-426). Within aa 390-516, human Collybistin shares 97% and 57% aa sequence identity with mouse and rat Collybistin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Collybistin/ARHGEF9 Antibody (MAB7848)(1)

Publication using MAB7848

• Sung-Jin Lee Presynaptic neuronal pentraxin receptor organizes excitatory and inhibitory synapses J. Neurosci, 2016-12-16;0(0):. 2016-12-16 [PMID: 27986928] (ICC, Human)

Bioinformatics

• Gene Symbol: ARHGEF9
• Uniprot:
  • O43307(Human)