Glutathione Synthetase Antibody (OTI1B8)

Western Blot: Glutathione Synthetase Antibody (1B8) [NBP2-03351] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glutathione Synthetase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Glutathione Synthetase.

Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (1B8) [NBP2-03351] Staining of paraffin-embedded Human prostate tissue using anti-Glutathione Synthetase mouse monoclonal antibody.

Flow Cytometry: Glutathione Synthetase Antibody (1B8) [NBP2-03351] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Glutathione Synthetase antibody, and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (1B8) [NBP2-03351] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Glutathione Synthetase mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (1B8) [NBP2-03351] - Staining of paraffin-embedded Human Kidney tissue using anti-Glutathione Synthetase mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC
• Clone: OTI1B8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.51 mg/ml

Glutathione Synthetase Antibody (OTI1B8) Summary

• Immunogen: Full length human recombinant protein of human GSS (NP_000169) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GSS
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 52.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.51 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Glutathione Synthetase Antibody (OTI1B8)

• EC 6.3.2.3
• Glutathione synthase
• glutathione synthetase
• GSH synthetase
• GSHS
• GSH-S
• MGC14098

Background

Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glutathione Synthetase Antibody (NBP2-03351)(3)

Publications using NBP2-03351

• Yoon SJ, Combs JA, Falzone A et al. Comprehensive Metabolic Tracing Reveals the Origin and Catabolism of Cysteine in Mammalian Tissues and Tumors Cancer Research 2023-05-02 [PMID: 36862034] (Immunohistochemistry, Human)
• Kang Y, Mockabee-Macias A, Jiang C et al. Non-canonical glutamate-cysteine ligase activity protects against ferroptosis Cell Metab 2020-12-28 [PMID: 33357455]
• Kraft AM, Petty HR Spatial locations of certain enzymes and transporters within pre-invasive ductal epithelial cells predict human breast cancer recurrences Am. J. Physiol., Cell Physiol. 2020-09-09 [PMID: 32903032] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: GSS
• Entrez:
  • 2937(Human)