Glutathione Synthetase Antibody (12F2) - BSA Free

Western Blot: Glutathione Synthetase Antibody (12F2) [NBP2-59455] - Lane 1: MDA-MB231 cell lysate, Lane 2: WiDr cell lysate, Lane 3: SW480 cell lysate, Lane 4.: A427 cell lysate, Lane 5: MCF7 cell lysate Lane 6: 293T cell lysate, Lane 7: HeLa cell lysate, Lane 8: HT1080 cell lysate Lane 9: U87MG cell lysate, Lane 10: A549 cell lysate

Immunocytochemistry/Immunofluorescence: Glutathione Synthetase Antibody (12F2) [NBP2-59455] - Analysis of GSS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GSS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of GSS in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GSS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 12F2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Glutathione Synthetase Antibody (12F2) - BSA Free Summary

• Description: Novus Biologicals Mouse Glutathione Synthetase Antibody (12F2) - BSA Free (NBP2-59455) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human Glutathione Synthetase (1-474aa) purified from E. coli
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GSS
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Glutathione Synthetase Antibody (12F2) - BSA Free

• EC 6.3.2.3
• Glutathione synthase
• glutathione synthetase
• GSH synthetase
• GSHS
• GSH-S
• MGC14098

Background

Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage byfree radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as ahomodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion ofgamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.(provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GSS