Glutathione S Transferase kappa 1 Antibody (16A11.E4.E3.E7) - BSA Free

SDS-Page: Glutathione S Transferase kappa 1 Antibody (16A11.E4.E3.E7) [NBP2-44172] - SDS-PAGE of Mouse anti-GSTK1 Monoclonal Antibody. Lane 1: Reduced Mouse anti-GSTK1 Monoclonal Antibody. Lane 2: 3 UL OPAL Pre-stained Marker Lane 3: Non-reduced Mouse anti-GSTK1 Monoclonal Antibody. Load: 1 Ug per lane. Predicted/Observed size: Non-reduced at 160 kDa/observed at 180-200 kDa; Reduced at 55, 25 kDa. Non-reduced migrates at slightly higher molecular weight.

Western Blot of Mouse anti-GSTK1 Monoclonal Antibody. Lane 1: Recombinant GSTK1 protein. Lane 2: GST. Load: 50 ng per lane. Primary antibody: Mouse anti-Glutathione S Transferase kappa 1 Antibody (16A11.E4.E3.E7)at 1:1,000 overnight at 4C. Secondary antibody: HRP Mouse Secondary Antibody at 1:40,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 27 kDa, 27 kDa for GSTK1.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC, IP, PAGE
• Clone: 16A11.E4.E3.E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Glutathione S Transferase kappa 1 Antibody (16A11.E4.E3.E7) - BSA Free Summary

• Description: This product is purified from roller bottle culture by Protein A chromatography followed by extensive dialysis against the buffer stated above
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This Protein A purified Glutathione S Transferase kappa 1 Antibody (16A11.E4.E3.E7) was prepared by repeated immunizations in mice with Glutathione S Transferase kappa 1 Protein. (Uniprot: Q9Y2Q3)
• Specificity: Reacts specifically with Glutathione S Transferase kappa 1 protein.
• Isotype: IgG2a Lambda
• Clonality: Monoclonal
• Host: Mouse
• Gene: GSTK1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:50000-1:200000
  • Immunohistochemistry 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • SDS-Page 1:100-1:2000
  • Western Blot 1:500-1:1000
• Application Notes: This product is tested by in ELISA, SDS-Page, and Western Blot. Suitable for most immunological techniques requiring high titer binding and lot-to-lot consistency. Specific conditions for reactivity should be optimized by the end user.

Reactivity Notes

Cross reactivity from other sources has not been determined

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Alternate Names for Glutathione S Transferase kappa 1 Antibody (16A11.E4.E3.E7) - BSA Free

• EC 2.5.1.18
• glutathione S-transferase k1
• glutathione S-transferase kappa 1
• glutathione S-transferase subunit 13 homolog
• Glutathione S-transferase subunit 13
• GST 13-13
• GST class-kappa
• GST
• GST13
• GST13-13
• GSTK1-1
• hGSTK1

Background

Glutathione transferases (GSTs) are a superfamily of enzymes that play a vital functional role in the cellular detoxification process. They catalyze the conjugation of the thiol group of glutathione (GSH) to the electrophilic groups of a wide range of hydrophobic substrates, leading to an easier removal of the latter from the cells (1) The Kappa class of GSTs comprises soluble enzymes originally isolated from the mitochondrial matrix of rats. Gsk1 catalyses some typical GST reactions, it has been proposed that it is structurally distinct from other classes of cytosolic GSTs (2). Gsk1 has been identified in rat, mouse, and human. The C terminus of Gsk1 was essential for localization of the protein to peroxisomes, and the C-terminal sequence Ala-Arg-Leu represents a peroxisome targeting signal (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GSTK1
• Uniprot:
  • Q9Y2Q3(Human)