Glutamate Receptor 4 Antibody (1059705) [Unconjugated]

HEK293 cells transfected with Human GRIA4 and eGFP (A) vs irrelevant and eGFP (B) was stained with Mouse Anti-Human GluR4 Monoclonal Antibody (Catalog # MAB113631) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

GluR4 was detected in immersion fixed Transfected HEK293 Human Embryonic Kidney Cell Line (Positive) and absent in Wild Type HEK293 Cell Line (Negative) using Mouse Anti-Human GluR4 Monoclonal Antibody (Catalog # MAB113631) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: Flow, ICC/IF
• Clone: 1059705
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Glutamate Receptor 4 Antibody (1059705) [Unconjugated] Summary

• Immunogen: Synthetic peptide
  Accession # P48058
• Specificity: Detects human GluR4 in direct ELISA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glutamate Receptor 4 Antibody (1059705) [Unconjugated]

• AMPA-selective glutamate receptor 4
• GluA4
• GluR4
• GluR-4
• GLUR4C
• GLUR4gluR-D
• GLURD
• GluR-D
• glutamate receptor 4
• Glutamate receptor ionotropic, AMPA 4
• glutamate receptor, ionotrophic, AMPA 4

Background

Ionotropic glutamate receptor 4 (GluR4) is a 100.9 kDa protein that is part of a family (iGluR 1-7) of ligand-gated ion channels that mediates fast transmission of neurotransmitter signals in neuronal cells. These receptors respond to several agonists including N-methyl-d-aspartic acid (NMDA), alpha -amino-3-hydroxyl-5-methylisoxazole-4-proprionic acid (AMPA), or kainic acid (KA). iGluRs are tetrameric structures made by the dimerization of dimers. These receptors can form through a combination of iGluR1-iGluR4 subunits. Mature human GluR4 is a 902 amino acid (aa) molecule, and alternate gene-splicing results in transcript variations encoding different isoforms that may effect their signal transduction properties. GluR4 is made up of an extracellular amino-terminal domain (ATD), ligand binding domain (LBD), a common pore-forming transmembrane domain (TMD) and an intracellular C-terminal domain (CTD).  GluR4's clam-shell shaped LBD binds to agonists. The LBD closes upon ligand binding to induce a conformational change to the TMD, resulting in the ion channel opening. GluR4 is highly expressed in areas that utilize fast kinetics and rapid desensitization. Examples of GluR4 expression include tissues of the central cervical nucleus in rats, the outer plexiform layer of goldfish, the retinal cells of chicken embryos and the human cerebral cortex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P48058(Human)