Glut1 Antibody (202915) [Phycoerythrin]

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Glut1 PE-conjugated Monoclonal Antibody (Catalog # FAB1418P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Jurkat human acute T cell leukemia cell line either (A) untreated or (B) cultured in nutrient-depleted media was stained with Mouse Anti-Human Glut1 PE-conjugated Monoclonal Antibody (Catalog # FAB1418P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 202915
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

Glut1 Antibody (202915) [Phycoerythrin] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human Glut1
  Met1-Val492
  Accession # AAA52571
• Specificity: Detects human Glut1. Stains human Glut1-transfected NS0 cells, but not NS0 control transfectants. Although Human Glut1 Antibody detects Glut1 on the surface of T cells (1, 2), it does not detect it on erythrocytes (5). The reason for this discrepancy is not understood, but may be related to conformational or post-translational modification differences.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC2A1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  12 months from date of receipt, 2 to 8 degreesC as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glut1 Antibody (202915) [Phycoerythrin]

• Choreoathetosis/Spasticity, Episodic (Paroxysmal Choreoathetosis)
• CSE
• DYT17
• DYT18
• DYT9
• EIG12
• Glucose transporter type 1, erythrocyte/brain
• Glut1
• GLUT-1
• GLUT1DS
• HepG2 glucose transporter
• HTLVR
• Human T-Cell Leukemia Virus (I and II) Receptor
• MGC141895
• MGC141896
• PED
• SLC2A1
• solute carrier family 2 (facilitated glucose transporter), member 1
• Solute Carrier Family 2 Member 1
• solute carrier family 2, facilitated glucose transporter member 1

Background

Glut1 belongs to the facilitative glucose transport protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains and is expressed at variable levels in many tissues including brain endothelial cells, CD8 + T cells, and erythrocytes (1-4). Glut1 is a major glucose transporter that mediates glucose transport across the mammalian blood-brain barrier.

1. Mueckler, M. et al. 1994, Eur. J. Biochem. 219:713.
2. Meuckler, M. et al. 1985, Science 229:941.
3. Jones, K.S. et al. 2006, J. Virol. 8291.
4. Takenouchi, N. et al. 2007, J. Virol. 1506.
5. Kinet, S. et al. 2007, Retrovirology 4:31.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glut1 Antibody (FAB1418P)(11)

Publications using FAB1418P

• Picard, LK;Niemann, JA;Littwitz-Salomon, E;Waldmann, H;Watzl, C; Restriction of Glycolysis Increases Serial Killing Capacity of Natural Killer Cells International journal of molecular sciences 2024-03-02 [PMID: 38474166] (Flow Cytometry, Human)
• LK Picard, E Littwitz-S, H Waldmann, C Watzl Inhibition of Glucose Uptake Blocks Proliferation but Not Cytotoxic Activity of NK Cells Cells, 2022-11-03;11(21):. 2022-11-03 [PMID: 36359883] (Flow Cytometry, Human)
• Tiffany R. Butterfield, David B. Hanna, Robert C. Kaplan, Xiaonan Xue, Jorge R. Kizer, Helen G. Durkin et al. Elevated CD4 + T-cell glucose metabolism in HIV+ women with diabetes mellitus AIDS 8/1/2022 [PMID: 35727147]
• B Theall, J Stampley, E Cho, J Granger, NM Johannsen, BA Irving, G Spielmann Impact of acute exercise on peripheral blood mononuclear cells nutrient sensing and mitochondrial oxidative capacity in healthy young adults Physiological Reports, 2021-12-01;9(23):e15147. 2021-12-01 [PMID: 34889067] (Flow Cytometry, Human)
• JL Marín Fran, M Genoula, D Corral, G Duette, M Ferreyra, M Maio, MB Dolotowicz, OE Aparicio-T, E Patiño-Mar, A Charton, A Métais, F Fuentes, V Soldan, EJ Moraña, D Palmero, M Ostrowski, P Schierloh, C Sánchez-To, R Hernández-, J Pedraza-Ch, Y Rombouts, D Hudrisier, E Layre, C Vérollet, I Maridonnea, O Neyrolles, MDC Sasiain, G Lugo-Villa, L Balboa Host-Derived Lipids from Tuberculous Pleurisy Impair Macrophage Microbicidal-Associated Metabolic Activity Cell Reports, 2020-12-29;33(13):108547. 2020-12-29 [PMID: 33378679] (Flow Cytometry, Human)
• LM Sanmarco, N Eberhardt, G Bergero, LP Quebrada P, PM Adami, LM Visconti, ÁR Minguez, Y Hernández-, EAC Silva, L Morelli, M Postan, MP Aoki Monocyte glycolysis determines CD8+ T cell functionality in human Chagas disease JCI Insight, 2019-09-19;4(18):. 2019-09-19 [PMID: 31479429] (Flow Cytometry, Human)
• Hugo Pontes Antunes, Ricardo Teixo, João André Carvalho, Miguel Eliseu, Inês Marques, Ana Mamede et al. Diabetes mellitus and prostate cancer metabolism: Is there a relationship? Archivio Italiano di Urologia e Andrologia 9/30/2018 [PMID: 30362687]
• F Venet, J Demaret, BJ Blaise, C Rouget, T Girardot, E Idealisoa, T Rimmelé, F Mallet, A Lepape, J Textoris, G Monneret IL-7 Restores T Lymphocyte Immunometabolic Failure in Septic Shock Patients through mTOR Activation J. Immunol., 2017-07-19;0(0):. 2017-07-19 [PMID: 28724580] (Flow Cytometry, Human)
• Tiffany R. Butterfield, David B. Hanna, Robert C. Kaplan, Jorge R. Kizer, Helen G. Durkin, Mary A. Young et al. Increased glucose transporter-1 expression on intermediate monocytes from HIV-infected women with subclinical cardiovascular disease AIDS 1/14/2017 [PMID: 27835618]
• Prost S, Relouzat F, Spentchian M, Ouzegdouh Y, Saliba J, Massonnet G, Beressi J, Verhoeyen E, Raggueneau V, Maneglier B, Castaigne S, Chomienne C, Chretien S, Rousselot P, Leboulch P Erosion of the chronic myeloid leukaemia stem cell pool by PPARgamma agonists. Nature, 2015-09-02;525(7569):380-3. 2015-09-02 [PMID: 26331539] (Flow Cytometry, Human)
• Piotrowski , Joshua T, Gomez , Timothy, Schoon , Renee A, Mangalam , Ashutosh, Billadeau , Daniel D WASH knockout T cells demonstrate defective receptor trafficking, proliferation, and effector function. Mol Cell Biol, 2012-12-28;33(5):958-73. 2012-12-28 [PMID: 23275443] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: SLC2A1
• Uniprot:
  • AAA52571()