Glucosylceramidase/GBA Antibody

Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody [NBP1-83389] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody [NBP1-83389] - Staining of human pancreas shows moderate granular cytoplasmic positivity in islets of Langerhans.

Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody [NBP1-83389] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody [NBP1-83389] - Staining of human prostate shows moderate granular cytoplasmic positivity in glandular cells.

Product Discontinued

Glucosylceramidase/GBA Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: YSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHTGTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYN
• Predicted Species: Rat (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GBA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Glucosylceramidase/GBA Antibody

• Acid beta-glucosidase
• Alglucerase
• beta-glucocerebrosidase
• D-glucosyl-N-acylsphingosine glucohydrolase
• EC 3.2.1.45
• GBA
• GBA1
• GC
• GCB
• GLUC
• glucosidase, beta, acid
• glucosidase, beta; acid (includes glucosylceramidase)
• Glucosylceramidase
• Imiglucerase
• lysosomal glucocerebrosidase

Background

GBA encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants encoding the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GBA1