Recombinant Human Glucokinase/GCK Protein

Images

 
SDS-Page: Recombinant Human Glucokinase/GCK Protein [NBP1-99787] - 10% SDS-PAGE gel Lane 1. 10 ug Pancreatic glucokinase. Lane 2. Protein MW marker.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, HPLC
Concentration
39.3 mg/ml

Order Details

Recombinant Human Glucokinase/GCK Protein Summary

Description
A recombinant protein corresponding to 470 amino acids of Human pancreas Glucokinase/GCK

Source: E. coli.

Protein/Peptide Type
Recombinant Protein
Gene
GCK
Purity
>80%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
53.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
50 mM potassium phosphate pH 7.4, 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, and 2.5% glycerol.
Concentration
39.3 mg/ml
Purity
>80%, by SDS-PAGE

Alternate Names for Recombinant Human Glucokinase/GCK Protein

  • EC 2.7.1
  • EC 2.7.1.2
  • FGQTL3
  • GCK
  • GK
  • GLK
  • glucokinase (hexokinase 4)
  • Glucokinase
  • Hexokinase-4
  • Hexokinase-D
  • HHF3
  • HK4
  • HK4pancreatic isozyme
  • HKIV
  • HXKP
  • LGLK
  • maturity onset diabetes of the young 2
  • MODY2

Background

Glucose is phosphorylated to glucose-6-phosphate by glucokinases. This gene is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The main function of this gene is to regulate carbohydrate metabolism. Recombinant human pancreatic Glucokinase (Accession# NP_000153)) has a C-terminal FLAG tag and has 470 amino acid residues. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol GCK
Entrez