Recombinant Human Glucokinase/GCK Protein

Product Discontinued

Recombinant Human Glucokinase/GCK Protein Summary

• Description: Recombinant protein containing 474 amino acids for Human Glucokinase/GCK
  
  

  Source:E. coli.
  Amino Acid Sequence:(P35557)

• Preparation Method: Determined to be >77% pure by SDS-PAGE
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: GCK
• Purity: >77%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30oC. Assay conditions: 25 mM HEPES pH 7.5 2 mM MgCl2 1.0 mM DTT 0.5 mM NADP 2.0 mM ATP 25 mM glucose 100ug/ml BSA 20 units/ml glucose 6-phosphate dehydrogenase and 10 nM human pancreatic glucokinase at 30oC for 30 min.
  Specific activity: 662 pmol/min/ug. One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30C. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2 mM ATP, 25 mM glucose, 100 ug/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human liver glucokinase at 30C for 30 min.
• Theoretical MW: 53.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM potassium phosphate pH 7.4, 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, and 2.5% glycerol.
• Concentration: 1 mg/ml
• Purity: >77%, by SDS-PAGE

Alternate Names for Recombinant Human Glucokinase/GCK Protein

• EC 2.7.1
• EC 2.7.1.2
• FGQTL3
• GCK
• GK
• GLK
• glucokinase (hexokinase 4)
• Glucokinase
• Hexokinase-4
• Hexokinase-D
• HHF3
• HK4
• HK4pancreatic isozyme
• HKIV
• HXKP
• LGLK
• maturity onset diabetes of the young 2
• MODY2

Background

Glucose is phosphorylated to glucose-6-phosphate by glucokinases. This gene is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The main function of this gene is to regulate carbohydrate metabolism. Recombinant human liver Glucokinase (Accession# NP_277042)) has a C-terminal FLAG tag and has 474 amino acid residues. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: GCK