Recombinant Human Glucokinase/GCK Protein

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SDS-Page: Recombinant Human Glucokinase/GCK Protein [NBP1-99786]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human Glucokinase/GCK Protein Summary

Description
Recombinant protein containing 474 amino acids for Human Glucokinase/GCK

Source:E. coli.
Amino Acid Sequence:(P35557)

Preparation
Method
Determined to be >77% pure by SDS-PAGE
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
GCK
Purity
>70% SDS-PAGE

Applications/Dilutions

Application Notes
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30oC. Assay conditions: 25 mM HEPES pH 7.5 2 mM MgCl2 1.0 mM DTT 0.5 mM NADP 2.0 mM ATP 25 mM glucose 100ug/ml BSA 20 units/ml glucose 6-phosphate dehydrogenase and 10 nM human pancreatic glucokinase at 30oC for 30 min.
Specific activity: 662 pmol/min/ug. One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30C. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2 mM ATP, 25 mM glucose, 100 ug/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human liver glucokinase at 30C for 30 min.
Theoretical MW
53.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
50 mM potassium phosphate pH 7.4, 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, and 2.5% glycerol.
Concentration
1 mg/ml
Purity
>70% SDS-PAGE

Alternate Names for Recombinant Human Glucokinase/GCK Protein

  • EC 2.7.1
  • EC 2.7.1.2
  • FGQTL3
  • GCK
  • GK
  • GLK
  • glucokinase (hexokinase 4)
  • Glucokinase
  • Hexokinase-4
  • Hexokinase-D
  • HHF3
  • HK4
  • HK4pancreatic isozyme
  • HKIV
  • HXKP
  • LGLK
  • maturity onset diabetes of the young 2
  • MODY2

Background

Glucose is phosphorylated to glucose-6-phosphate by glucokinases. This gene is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The main function of this gene is to regulate carbohydrate metabolism. Recombinant human liver Glucokinase (Accession# NP_277042)) has a C-terminal FLAG tag and has 474 amino acid residues. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol GCK
Entrez