Glucokinase/GCK Antibody

Western Blot: Glucokinase/GCK Antibody [NBP1-33144] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glucokinase antibody [N1C1-2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Glucokinase/GCK Antibody [NBP1-33144] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glucokinase protein stained by Glucokinase antibody [N1C1-2] diluted at 1:500.Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: Glucokinase/GCK Antibody [NBP1-33144] - MCF7 xenograft, using Glucokinase antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: Glucokinase/GCK Antibody [NBP1-33144] - Sample (30 ug of whole cell lysate) A: H1299 B: Hela 10% SDS PAGE; antibody diluted at 1:1000.

Western Blot: Glucokinase/GCK Antibody [NBP1-33144] - MCF7 xenograft, using Glucokinase antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunocytochemistry/Immunofluorescence: Glucokinase/GCK Antibody [NBP1-33144] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.

• Reactivity: Hu, Mu, Rt, Bv, Fe, Sh
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Glucokinase/GCK Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Glucokinase. The exact sequence is proprietary.
• Predicted Species: Mouse (97%), Rat (97%), Sheep (97%), Feline (96%), Bovine (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GCK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cat (96%), Zebrafish (86%), Xenopus laevis (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol (pH7).
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for Glucokinase/GCK Antibody

• EC 2.7.1
• EC 2.7.1.2
• FGQTL3
• GCK
• GK
• GLK
• glucokinase (hexokinase 4)
• Glucokinase
• Hexokinase-4
• Hexokinase-D
• HHF3
• HK4
• HK4pancreatic isozyme
• HKIV
• HXKP
• LGLK
• maturity onset diabetes of the young 2
• MODY2

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: GCK