Glucokinase/GCK Antibody

Western Blot: Glucokinase/GCK Antibody [H00002645-D01P] - Analysis of GCK expression in mouse liver.

Immunocytochemistry/Immunofluorescence: Glucokinase/GCK Antibody [H00002645-D01P] - Analysis of antibody to GCK on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: Glucokinase/GCK Antibody [H00002645-D01P] - Analysis of GCK expression in PC-12.

Western Blot: Glucokinase/GCK Antibody [H00002645-D01P] - Analysis of GCK expression in human spleen.

Western Blot: Glucokinase/GCK Antibody [H00002645-D01P] - Analysis of GCK expression in transfected 293T cell line by GCK polyclonal antibody.Lane 1: GCK transfected lysate(52.20 KDa).Lane 2: Non-transfected lysate.

Product Discontinued

Glucokinase/GCK Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: GCK (AAH01890.1, 1 a.a. - 465 a.a.) full-length human protein. MLDDRARMEAAKKEKVEQILAEFQLQEEDLKKVMRRMQKEMDRGLRLETHEEASVKMLPTYVRSTPEGSEVGDFLSLDLGGTNFRVMLVKVGEGEEGQWSVKTKHQMYSIPEDAMTGTAEMLFDYISECISDFLDKHQMKHKKLPLGFTFSFPVRHEDIDKGILLNWTKGFKASGAEGNNVVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDHQCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRLVDESSANPGQQLYEKLIGGKYMGELVRLVLLRLVDENLLFHGEASEQLRTRGAFETRFVSQVESDTGDRKQIYNILSTLGLRPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAALVSAVACKKACMLGQ
• Specificity: GCK - glucokinase (hexokinase 4, maturity onset diabetes of the young 2),
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GCK
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Glucokinase/GCK Antibody

• EC 2.7.1
• EC 2.7.1.2
• FGQTL3
• GCK
• GK
• GLK
• glucokinase (hexokinase 4)
• Glucokinase
• Hexokinase-4
• Hexokinase-D
• HHF3
• HK4
• HK4pancreatic isozyme
• HKIV
• HXKP
• LGLK
• maturity onset diabetes of the young 2
• MODY2

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GCK
• Entrez:
  • 2645(Human)
• Uniprot:
  • AAH01890.1(Human)