Glucocorticoid Receptor alpha Antibody (3F2)

Western Blot: Glucocorticoid Receptor alpha Antibody (3F2) [NBP2-75509] - Analysis of Glucocorticoid Receptor alpha on SiHa (1) and A549 (2) using anti-Glucocorticoid Receptor alpha antibody at 1/5,000 dilution.

Immunocytochemistry/Immunofluorescence: Glucocorticoid Receptor alpha Antibody (3F2) [NBP2-75509] - Analysis of Hela cells labeling Glucocorticoid Receptor alpha with Mouse anti-Glucocorticoid Receptor alpha antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Glucocorticoid Receptor alpha antibody at 1/50 dilution in 2% BSA overnight at 4 . Goat Anti-Mouse IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Immunohistochemistry-Paraffin: Glucocorticoid Receptor alpha Antibody (3F2) [NBP2-75509] - Analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-Glucocorticoid Receptor alpha antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: Glucocorticoid Receptor alpha Antibody (3F2) [NBP2-75509] - Analysis of A549 cells with Glucocorticoid Receptor alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.

Immunohistochemistry-Paraffin: Glucocorticoid Receptor alpha Antibody (3F2) [NBP2-75509] - Analysis of paraffin-embedded human spleen tissue using anti-Glucocorticoid Receptor alpha antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Glucocorticoid Receptor alpha Antibody (3F2) [NBP2-75509] - Analysis of paraffin-embedded mouse smooth muscle tissue using anti-Glucocorticoid Receptor alpha antibody. Counter stained with hematoxylin.

Product Discontinued

Glucocorticoid Receptor alpha Antibody (3F2) Summary

• Immunogen: Recombinant full length protein corresponding to Human Glucocorticoid Receptor alpha. (SwissProt: P04150 Human; SwissProt: P06537 Mouse)
• Localization: Cytoplasm. Nucleus.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NR3C1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000
• Theoretical MW: 86 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein G purified

Alternate Names for Glucocorticoid Receptor alpha Antibody (3F2)

• GCR
• glucocorticoid receptor
• GRGCCR
• GRLNuclear receptor subfamily 3 group C member 1
• nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
• nuclear receptor subfamily 3, group C, member 1

Background

Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the Glucocorticoid Receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain GR. The human GR exists in two forms, alpha and beta, which are thought to be the result of alternative splicing of a single gene. Sequence analysis indicates that alpha and beta forms of human GR are 777 and 742 amino acids long, respectively. They are identical up to residue 727, after which they diverge. After ligand binding, the 94 kDa GR alpha isoform translocates from the cytoplasm to the nucleus where it regulates gene expression. In contrast, the 90 kDa GR beta isoform does not appear to bind either glucocorticoid agonists or antagonists, and has been localized predominantly in the nucleus independent of hormone treatment in some human cell lines. Studies suggest that human GR alpha has a greater affinity for GR response elements (GREs) than GR beta only when in the ligand bound state.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NR3C1