Glucocorticoid Receptor alpha Antibody (3F1)

Western Blot: Glucocorticoid Receptor alpha Antibody (3F1) [NBP2-75508] - Analysis of Glucocorticoid Receptor alpha on SiHa (1) and A549 (2) cell lysates using anti-Glucocorticoid Receptor alpha antibody at 1/5,000 dilution.

Immunocytochemistry/Immunofluorescence: Glucocorticoid Receptor alpha Antibody (3F1) [NBP2-75508] - Staining Glucocorticoid Receptor alpha (green) in SiHa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Glucocorticoid Receptor alpha Antibody (3F1) [NBP2-75508] - Analysis of paraffin-embedded human spleen tissue using anti-Glucocorticoid Receptor alpha antibody. Counter stained with hematoxylin.

Flow Cytometry: Glucocorticoid Receptor alpha Antibody (3F1) [NBP2-75508] - Analysis of A549 cells with Glucocorticoid Receptor alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.

Immunohistochemistry-Paraffin: Glucocorticoid Receptor alpha Antibody (3F1) [NBP2-75508] - Analysis of paraffin-embedded human breast cancer tissue using anti-Glucocorticoid Receptor alpha antibody. Counter stained with hematoxylin.

Product Discontinued

Glucocorticoid Receptor alpha Antibody (3F1) Summary

• Immunogen: Recombinant full length protein corresponding to Human Glucocorticoid Receptor alpha. (SwissProt: P04150 Human)
• Localization: Nucleus. Cytoskeleton. Mitochondrion.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: NR3C1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:5000
• Theoretical MW: 86 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein G purified

Alternate Names for Glucocorticoid Receptor alpha Antibody (3F1)

• GCR
• glucocorticoid receptor
• GRGCCR
• GRLNuclear receptor subfamily 3 group C member 1
• nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
• nuclear receptor subfamily 3, group C, member 1

Background

Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the Glucocorticoid Receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain GR. The human GR exists in two forms, alpha and beta, which are thought to be the result of alternative splicing of a single gene. Sequence analysis indicates that alpha and beta forms of human GR are 777 and 742 amino acids long, respectively. They are identical up to residue 727, after which they diverge. After ligand binding, the 94 kDa GR alpha isoform translocates from the cytoplasm to the nucleus where it regulates gene expression. In contrast, the 90 kDa GR beta isoform does not appear to bind either glucocorticoid agonists or antagonists, and has been localized predominantly in the nucleus independent of hormone treatment in some human cell lines. Studies suggest that human GR alpha has a greater affinity for GR response elements (GREs) than GR beta only when in the ligand bound state.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NR3C1