Glucagon R/GCGR Antibody

Images

 
Western Blot: Glucagon R/GCGR Antibody [NBP1-30971] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Glucagon Receptor antibody at a dilution of 1:1000.
Immunohistochemistry-Paraffin: Glucagon R/GCGR Antibody [NBP1-30971] - Paraffin-embedded CL1-0 xenograft, using antibody at 1:100 dilution.
Western Blot: Glucagon R/GCGR Antibody [NBP1-30971] - Sample (30 ug of whole cell lysate)A: 293T 10% SDS PAGE, antibody diluted at 1:1000.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Glucagon R/GCGR Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Glucagon Receptor. The exact sequence is proprietary.
Localization
Cell membrane, Multi-pass membrane protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GCGR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Mouse (87%), Rat (87%), Bovine (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Glucagon R/GCGR Antibody

  • FLJ97182
  • GCGR
  • GGR
  • GLR
  • GL-R
  • Glucagon R
  • glucagon receptor
  • GlucagonR
  • MGC138246

Background

Glucagon, a pancreatic hormone, functions as an antagonist to insulin, stimulating the conversion of glycogen to glucose and increasing blood sugar levels. GLP-1 functions as a transmitter in the central nervous system, inhibiting feeding and drinking behavior. Both glucagon and GLP-1 function through their specific binding to the glucagon receptor or GLP-1R, respectively. The glucagon receptor shows expression in liver, kidney and adipose tissue. The 56 GLP-1R expression primarily localizes to areas of the hypothalamus involved in feeding behavior. Both receptors and their ligands serve as potential targets for the therapeutic treatment of diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GCGR
Entrez
Uniprot