Glucagon R/GCGR Antibody

Product Discontinued

Glucagon R/GCGR Antibody Summary

• Immunogen: Peptide with sequence C-HGPPSKELQFGR corresponding to internal region (near C-Terminus) according to NP_000151.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GCGR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Preliminary experiments gave an approx. 35 kDa band in human kidney and liver lysates after 0.03 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 54.0 kDa band according to NP_000151.1. The 35 kDa band was successfully blocked by incubation with the immunizing peptide.
• Control:

  Glucagon R/GCGR Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for Glucagon R/GCGR Antibody

• FLJ97182
• GCGR
• GGR
• GLR
• GL-R
• Glucagon R
• glucagon receptor
• GlucagonR
• MGC138246

Background

Glucagon, a pancreatic hormone, functions as an antagonist to insulin, stimulating the conversion of glycogen to glucose and increasing blood sugar levels. GLP-1 functions as a transmitter in the central nervous system, inhibiting feeding and drinking behavior. Both glucagon and GLP-1 function through their specific binding to the glucagon receptor or GLP-1R, respectively. The glucagon receptor shows expression in liver, kidney and adipose tissue. The 56 GLP-1R expression primarily localizes to areas of the hypothalamus involved in feeding behavior. Both receptors and their ligands serve as potential targets for the therapeutic treatment of diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glucagon R/GCGR Antibody (NBP1-00194)(1)

Publication using NBP1-00194

• Sorensen H, Winzell MS, Brand CL et al. Glucagon receptor knockout mice display increased insulin sensitivity and impaired beta-cell function. Diabetes 2006-12-01 [PMID: 17130493]

Bioinformatics

• Gene Symbol: GCGR
• Entrez:
  • 2642(Human)
• Uniprot:
  • NP_000151.1(Human)