GLP1 Antibody (10) [Biotin]

Product Discontinued

GLP1 Antibody (10) [Biotin] Summary

• Immunogen: Synthetic GLP-1(7-17)
• Epitope: Free N-terminus of GLP-1(7-37) and GLP-1(7-36) amide.
• Specificity: Binds the free N-terminus of GLP-1(7-37) and GLP-1(7-36)amide and shows <0.2% cross-reactivity with GLP-1(9-36)amide, glucagon and human GIP. Cross-reacts 0.25% with GLP-1(1-37), approximately 6% with human GLP-2 and approximately 50% with exendin-4. Cross-reacts with Liraglutide.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GCG
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Sandwich ELISA Detection
• Application Notes: It reacts in ELISA with GLP-1(7-37) coated directly onto the microtiter well. It binds free exendin-4 in solution and can function as a capture antibody for exendin-4. Results show detection limits of 44pmol/L which is 10-20 times higher than the basal concentration of GLP-1, so the assays have to be optimized.
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Reactivity Notes

The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10 mM Phosphate buffer (pH 7.4) and 0.5 M NaCl
• Preservative: 0.09% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for GLP1 Antibody (10) [Biotin]

• glicentin-related polypeptide
• GLP1
• GLP-1
• glucagon-like peptide 1
• GRPP

Background

Glucagon-like peptide 1(7-36)amide (GLP-1(7-36)amide) is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GCG