| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | NL1249R | |||
| Carrier Free | MAB1249-SP | |||
| Carrier Free | IC1249V-100UG | |||
| Carrier Free | IC1249U-100UG | |||
| Carrier Free | IC1249T-100UG | |||
| Carrier Free | IC1249S-100UG | |||
| Carrier Free | IC1249R-100UG | |||
| Carrier Free | IC1249N-100UG | |||
| Carrier Free | IC1249G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-21803-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38329-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-23558-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38330-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05180-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-23591-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-21747-0.025mg | |||
![Immunocytochemistry/Immunofluorescence: Glucagon Antibody (09) [NBP2-21803] - Staining in human pancreas tissueSignal is strong in alpha cells. Dilution: 1:4000](http://images.novusbio.com/fullsize/Glucagon-Antibody-09-Immunocytochemistry-Immunofluorescence-NBP2-21803-img0001.jpg "Immunocytochemistry/Immunofluorescence: Glucagon Antibody (09) [NBP2-21803] - Staining in human pancreas tissueSignal is strong in alpha cells. Dilution: 1:4000")
![Immunohistochemistry-Paraffin: Glucagon Antibody (09) [NBP2-21803] - Staining of human pancreas tissue . Alpha cells of the Langerhans islet were stained strongly. Dilution: 1:8000](http://images.novusbio.com/fullsize/Glucagon-Antibody-09-Immunohistochemistry-Paraffin-NBP2-21803-img0002.jpg)
![Sandwich ELISA: Glucagon Antibody (09) - BSA Free [NBP2-21803] - Glucagon Antibody (09) [NBP2-21803] - The calibration curve of a sandwich assay for Glucagon using NBP2-21803 as the capture antibody and NBP2-21802B as the biotinylated detection antibody.](http://images.novusbio.com/fullsize/Glucagon-Antibody-09-Sandwich-ELISA-NBP2-21803-img0009.jpg)
![Immunohistochemistry: Glucagon Antibody (K79bB10) [NB600-1506] - Mouse pancreatic islet alpha cells were stained using Monoclonal Anti-Glucagon, clone no. K79bB10, Anti-Mouse IgG-peroxidase and AEC substrate (red). The tissue was counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize/Glucagon-Antibody-K79bB10-Immunohistochemistry-NB600-1506-img0001.jpg "Immunohistochemistry: Glucagon Antibody (K79bB10) [NB600-1506] - Mouse pancreatic islet alpha cells were stained using Monoclonal Anti-Glucagon, clone no. K79bB10, Anti-Mouse IgG-peroxidase and AEC substrate (red). The tissue was counterstained with hematoxylin (blue).")
![Immunoprecipitation: Glucagon Antibody [NBP3-23954] - Immunofluorescence of Glucagon Antibody. Cell Line: MCF7 cells. Fixative: 100% Methanol. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-Glucagon at 15µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG DyLight™488 at 5µL/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A). DAPI. (B). Anti-Glucagon + DyLight™488 secondary. (C). Merge A+B. (D). secondary only. Localization expected: Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-23954_rabbit-glucagon-pab-231202523584713.jpg "Immunoprecipitation: Glucagon Antibody [NBP3-23954] - Immunofluorescence of Glucagon Antibody. Cell Line: MCF7 cells. Fixative: 100% Methanol. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-Glucagon at 15µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG DyLight™488 at 5µL/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A). DAPI. (B). Anti-Glucagon + DyLight™488 secondary. (C). Merge A+B. (D). secondary only. Localization expected: Cytoplasm.")
![ELISA: Glucagon Antibody [NBP3-23954] - ELISA Results of Glucagon Antibody. Each well was coated with 1µg of conjugate. The starting concentration of antibody in the dilution series was 5 µg/ml. The titer is 1:34,800 Glucagon - Free peptide [Green Line], 1:47200 Glucagon Standard - BSA conjugated [Blue Line], and 1:20,500 Glucagon - BSA conjugated [Purple Line]. Each point on the Y-axis represents a 3-fold dilution. 3% Fish Gel, HRP conjugated Goat anti-Rabbit IgG (H&L), and TMB substrate were used for detection.](http://images.novusbio.com/fullsize/nbp3-23954_rabbit-glucagon-pab-23120252358477.jpg)
![Immunocytochemistry/ Immunofluorescence: Glucagon Antibody [NBP3-23954] - Immunofluorescence of Glucagon Antibody. Cell Line: NIH/3T3 cells. Fixative: 100% Methanol. Permeabilization: Triton X-100. Primary Antibody: Anti-Glucagon at 15µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG DyLight™488 at 5µL/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A). DAPI. (B). Anti-Glucagon + DyLight™488 secondary. (C). Merge A+B. (D). secondary only. Localization expected: Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-23954_rabbit-glucagon-pab-2312025235983.jpg)
![Immunocytochemistry/ Immunofluorescence: Glucagon Antibody [NBP3-23954] - Immunofluorescence of Glucagon Antibody. Cell Line: U20S cells. Fixative: 4% PFA. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-Glucagon at 15µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG DyLight™488 at 5µL/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A). DAPI. (B). Anti-Glucagon + DyLight™488 secondary. (C). Merge A+B. (D). secondary only. Localization expected: Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-23954_rabbit-glucagon-pab-23120252358471.jpg)
![Immunocytochemistry/ Immunofluorescence: Glucagon Antibody [NBP3-23954] - Immunofluorescence of Glucagon Antibody. Cell Line: NIH/3T3 cells. Fixative: 4% PFA. Permeabilization: Triton X-100. Primary Antibody: Anti-Glucagon at 15µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG DyLight™488 at 5µL/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A). DAPI. (B). Anti-Glucagon + DyLight™488 secondary. (C). Merge A+B. (D). secondary only. Localization expected: Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-23954_rabbit-glucagon-pab-231202523584747.jpg)
![Western Blot: Glucagon Antibody [NBP3-23954] - Western Blot of Glucagon Antibody. Lane 1: Opal Prestained Molecular Weight. Lane 2: COS-7 Lysate - reduced (20µg). Lane 3: BSA Conjugated Glucagon peptide - reduced (0.02µg).Lane 4: Insulin - reduced (0.05µg). Primary Antibody: Anti-Glucagon [Rabbit] Antibody at 1.0µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG (MX10) Peroxidase conjugated at 1:70,000 for 30mins at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 1hr at RT.Expected MW: ~21kDa. Observed MW: endogenous detection in COS-7 Lysate at ~35kDa. Glucagon peptide is detected at the MW of BSA. No cross-reactivity with insulin is observed.Exposure: 25 sec.](http://images.novusbio.com/fullsize/nbp3-23954_rabbit-glucagon-pab-231202523584717.jpg)
![Immunohistochemistry: Glucagon Antibody [NBP3-23954] - Immunohistochemistry results using Glucagon Antibody. Tissue: alpha cells in CD1 mouse pancreatic islets. Fixation: 4% paraformaldehyde. Antigen Retrieval: 10mM Sodium Citrate buffer for 10 mins at 95-100°C. Blocking: PBS, 1% ovalbumin, 0.3% Triton X-100. Primary Antibody: Anti-Glucagon at 1:100 overnight at RT. Secondary Antibody: Anti-Rabbit Alexa Fluor 488 at 1:500 for 1hr at RT. Original magnification 20x. Courtesy of Prof. Merighi, University of Turin.](http://images.novusbio.com/fullsize/nbp3-23954_rabbit-glucagon-pab-231202523584723.jpg)
![Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38329] - Analysis in human pancreas and skeletal muscle tissues. Corresponding GCG RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Glucagon-Antibody-Immunohistochemistry-Paraffin-NBP2-38329-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38329] - Analysis in human pancreas and skeletal muscle tissues. Corresponding GCG RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38329] - Staining of human colon, pancreas, skeletal muscle and small intestine using Anti-GCG antibody NBP2-38329 (A) shows similar protein distribution across tissues to independent antibody NBP2-38330 (B).](http://images.novusbio.com/fullsize/Glucagon-Antibody-Immunohistochemistry-Paraffin-NBP2-38329-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Glucagon Antibody [NBP2-38329] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Glucagon-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38329-img0009.jpg)
![Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38329] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Glucagon-Antibody-Immunohistochemistry-Paraffin-NBP2-38329-img0014.jpg)
![Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38329] - Staining of human pancreas shows strong cytoplasmic positivity in endocrine glandular cells.](http://images.novusbio.com/fullsize/Glucagon-Antibody-Immunohistochemistry-Paraffin-NBP2-38329-img0012.jpg)
![Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38329] - Staining of human small intestine shows strong cytoplasmic positivity in enteroendocrine cells.](http://images.novusbio.com/fullsize/Glucagon-Antibody-Immunohistochemistry-Paraffin-NBP2-38329-img0013.jpg)
![Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38329] - Staining of human colon shows moderate cytoplasmic positivity in enteroendocrine cells.](http://images.novusbio.com/fullsize/Glucagon-Antibody-Immunohistochemistry-Paraffin-NBP2-38329-img0015.jpg)
![Immunohistochemistry: Glucagon Antibody (4) - BSA Free [NBP2-23558] - Endocrine alpha cells from diabetic mice stained with (1:500). Courtesy of Dr. Annette Plesner, University of British Columbia](http://images.novusbio.com/fullsize/GLP1-Antibody-4-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-23558-img0001.jpg "Immunohistochemistry: Glucagon Antibody (4) - BSA Free [NBP2-23558] - Endocrine alpha cells from diabetic mice stained with (1:500). Courtesy of Dr. Annette Plesner, University of British Columbia")
![Glucagon Antibody (4) - BSA Free [NBP2-23558] - BSA Free [NBP2-23558]](http://images.novusbio.com/fullsize/GLP1-Antibody-4-BSA-Free-NBP2-23558-img0002.jpg)
![Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38330] - Analysis in human pancreas and skeletal muscle tissues. Corresponding GCG RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Glucagon-Antibody-Immunohistochemistry-Paraffin-NBP2-38330-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38330] - Analysis in human pancreas and skeletal muscle tissues. Corresponding GCG RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38330] - Staining of human colon, pancreas, skeletal muscle and small intestine using Anti-GCG antibody NBP2-38330 (A) shows similar protein distribution across tissues to independent antibody NBP2-38329 (B).](http://images.novusbio.com/fullsize/Glucagon-Antibody-Immunohistochemistry-Paraffin-NBP2-38330-img0008.jpg)
![Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38330] - Staining of human pancreas shows strong cytoplasmic positivity in endocrine glandular cells.](http://images.novusbio.com/fullsize/Glucagon-Antibody-Immunohistochemistry-Paraffin-NBP2-38330-img0012.jpg)
![Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38330] - Staining of human colon shows strong cytoplasmic positivity in enteroendocrine cells.](http://images.novusbio.com/fullsize/Glucagon-Antibody-Immunohistochemistry-Paraffin-NBP2-38330-img0010.jpg)
![Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38330] - Staining of human small intestine shows strong cytoplasmic positivity in enteroendocrine cells.](http://images.novusbio.com/fullsize/Glucagon-Antibody-Immunohistochemistry-Paraffin-NBP2-38330-img0011.jpg)
![Immunohistochemistry-Paraffin: Glucagon Antibody [NBP2-38330] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Glucagon-Antibody-Immunohistochemistry-Paraffin-NBP2-38330-img0013.jpg)
![GLP1 Antibody (10) - BSA Free [NBP1-05180] - BSA Free [NBP1-05180]](http://images.novusbio.com/fullsize/GLP1-Antibody-10-BSA-Free-NBP1-05180-img0001.jpg "GLP1 Antibody (10) - BSA Free [NBP1-05180] - BSA Free [NBP1-05180]")
![ELISA: GLP1 Antibody (10) - BSA Free [NBP1-05180] - Calibration curve using NBP2-23506 as the capture antibody and NBP2-23557 as the biotinylated detection antibody. Image using the Biotin form of this antibody.](http://images.novusbio.com/fullsize/GLP1-Antibody-10-BSA-Free-ELISA-NBP1-05180-img0002.jpg)
![ELISA: GLP1 Antibody (10) - BSA Free [NBP1-05180] - By using the above monoclonal antibodies, it is possible to measure intact exendin-4 by a sandwich ELISA which is not subject to interference from GLP-1 or other glucagon-related peptides that may be present. NBP1-05180 is used as capture antibody in combination with a biotinylated detection antibody from the ABS 012 series. The best sensitivity in an unoptimized buffer assay was obtained with biotinylated ABS 012-35 as the detection antibody.](http://images.novusbio.com/fullsize/GLP1-Antibody-10-BSA-Free-ELISA-NBP1-05180-img0003.jpg)
![Glucagon Antibody (49) - BSA Free [NBP2-23591]](http://images.novusbio.com/fullsize/GLP1-Antibody-49-BSA-Free-NBP2-23591-img0001.jpg "Glucagon Antibody (49) - BSA Free [NBP2-23591]")
![Western Blot: Glucagon Antibody [NBP2-16672] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/Glucagon-Antibody-Western-Blot-NBP2-16672-img0002.jpg "Western Blot: Glucagon Antibody [NBP2-16672] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE gel, diluted at 1:500.")
![Glucagon Antibody (03) - BSA Free [NBP2-21747] - BSA Free [NBP2-21747]](http://images.novusbio.com/fullsize/GLP1-Antibody-03-BSA-Free-NBP2-21747-img0001.jpg "Glucagon Antibody (03) - BSA Free [NBP2-21747] - BSA Free [NBP2-21747]")
![ELISA: Glucagon Antibody (03) - BSA Free [NBP2-21747] - The calibration curve of a sandwich assay for Non-amidated GLP-1 using NBP2-21747 as the capture antibody and NBP1-05180B as the biotinylated detection antibody.](http://images.novusbio.com/fullsize/GLP1-Antibody-03-BSA-Free-ELISA-NBP2-21747-img0002.jpg)