Glis1 Antibody

Western Blot: GLIS1 Antibody [NBP2-16667] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: GLIS1 Antibody [NBP2-16667] - Immunofluorescence analysis of paraformaldehyde-fixed A549, using antibody at 1:500 dilution.

Product Discontinued

Glis1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Glis1. The exact sequence is proprietary.
• Localization: Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GLIS1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Glis1 Antibody

• FLJ36155
• GLIS family zinc finger 1
• Glis1
• GLI-similar 1
• zinc finger protein GLIS1

Background

GLIS1 is a GLI (MIM 165220)-related Kruppel-like zinc finger protein that functions as an activator and repressor of transcription (Kim et al., 2002 [PubMed 12042312]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: GLIS1
• Uniprot:
  • Q8NBF1(Human)