Glis1 Antibody [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 405

Glis1 Antibody [Alexa Fluor® 405] Summary

• Specificity: Detects human Glis1 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse Glis1 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glis1 Antibody [Alexa Fluor® 405]

• FLJ36155
• GLIS family zinc finger 1
• Glis1
• GLI-similar 1
• zinc finger protein GLIS1

Background

Glis1 (GLI/glioma-Similar 1; also GliH1) is a 66 kDa (predicted) member of the Kruppel-like zinc finger family of proteins. In the adult, it is principally expressed in placenta and kidney, while in the embryo, it has widespread expression in mesodermal structures. Glis1 can be induced in suprabasal keratinocytes by activating cytokines, and is believed to promote a unique differentiation program. Glis1 is also effective in generating iPSCs from somatic cells (fibroblasts) when concurrently expressed with Oct3, Sox2 and Klf4. Multiple reprogramming pathways are involved, and include Myc, Wnt and Nanog molecules. Functionally, Glis1 is believed to act as a transcriptional repressor.  Although it has been described as possessing an activation domain, it would appear that gene promotion is a consequence of the repression of a "repressor". The consequence sequence ACCACCCA corresponds to a GLIS binding motif. Human Glis1 is 620 amino acids (aa) in length. Based on mouse, it contains a zinc-finger repressor region (aa 197-346) plus a putative transactivation domain (aa 448-620). And again based on mouse, there might be an approximately 796 aa splice form that shows an alternative start site 171 aa upstream of the 620 aa isoform.  The existence of such an isoform would generate an approximately 95-100 kDa isoform in SDS-PAGE. Over aa 446-620, human Glis1 shares 80% aa sequence identity with mouse Glis1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.