GLI-3 Antibody [Alexa Fluor® 750]

• Reactivity: Hu, Mu
• Applications: WB, ChIP, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 750

GLI-3 Antibody [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human GLI-3
  Met1-Glu479
  Accession # P10071
• Specificity: Detects human and mouse GLI-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) GLI-1 and rhGLI-2 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GLI-3 Antibody [Alexa Fluor® 750]

• ACLS
• ACLSPAPAPHS
• ADD
• Bph
• GCPS
• GLI family zinc finger 3
• GLI3 form of 190 kDa
• GLI3 full length protein
• GLI3
• GLI-3
• GLI3-190
• GLI3FL
• GLI-Kruppel family member GLI3
• glioma-associated oncogene family zinc finger 3
• Greig cephalopolysyndactyly syndrome
• oncogene GLI3
• PAP-A
• PAPA1
• PAPB
• PAPBDNA-binding protein
• Pdn
• PHS
• PPDIV
• Xt
• zinc finger protein GLI3

Background

Human GLI-3 is a 190 kDa transcription factor of the C2H2-type zinc-finger protein family. GLI-3 appears to play a role in limb and brain development, probably through sonic hedgehog signaling. GLI-3 is regulated by PKA-mediated phosphorylation. Unphosphorylated full-length GLI-3 is a transcriptional activator, while phosphorylated GLI-3 is cleaved after amino acid 700 to generate an N-terminal 83 kDa transcriptional repressor. Over the region used as immunogen, human GLI-3 is 95% identical to the corresponding mouse and canine protein sequences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.