GLDC Antibody

Western Blot: GLDC Antibody [NBP1-32907] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membranes were blotted with Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: GLDC Antibody [NBP1-32907] - Hep G2 cells were fixed in ice-cold MeOH for 5 min. Green: Glycine dehydrogenase protein stained by Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:400. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-32907] - Paraffin-embedded mouse brain. Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:500.

Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-32907] - Paraffin-embedded mouse liver. Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:500.

Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-32907] - Paraffin-embedded rat brain. Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:500

Simple Western: GLDC Antibody [NBP1-32907] - Simple Western lane view shows a specific band for GLDC in 0.2 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunoprecipitation: GLDC Antibody [NBP1-32907] - Immunoprecipitation of Glycine dehydrogenase protein from HepG2 whole cell extracts using 5 ug of Glycine dehydrogenase antibody [N3C2-2], Internal. Western blot analysis was performed using Glycine dehydrogenase antibody [N3C2-2], Internal. EasyBlot anti-Rabbit IgG(was used as a secondary reagent.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, Simple Western, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

GLDC Antibody Summary

• Description: Novus Biologicals Rabbit GLDC Antibody (NBP1-32907) is a polyclonal antibody validated for use in IHC, WB, ICC/IF, Simple Western and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human GLDC. The exact sequence is proprietary.
• Localization: Mitochondrion
• Predicted Species: Bovine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GLDC
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Simple Western 1:200
  • Western Blot 1:500-1:3000
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HepG2 lysate 0.2 mg/mL, separated by Size, antibody dilution of 1:200, apparent MW was 111 kDa.
• Theoretical MW: 113 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (85%), Xenopus laevis (86%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for GLDC Antibody

• EC 1.4.4.2
• GCE
• GCSPMGC138198
• Glycine cleavage system P protein
• glycine cleavage system protein P
• glycine decarboxylase P-protein
• Glycine decarboxylase
• glycine dehydrogenase (decarboxylating)
• glycine dehydrogenase (decarboxylating; glycine decarboxylase, glycine cleavagesystem protein P)
• glycine dehydrogenase [decarboxylating], mitochondrial
• HYGN1
• MGC138200
• NKH

Background

The enzyme system for cleavage of glycine (glycine cleavage system; GCS; EC 2.1.2.10), which is confined to themitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase),H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (alipoamide dehydrogenase). Glycine encephalopathy (GCE; MIM 605899) may be due to a defect in any one of these enzymes;see MIM 238310, MIM 238330, and MIM 238331.(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GLDC