GITR/TNFRSF18 Overexpression Lysate (Native)

Images

 
Western Blot: GITR Overexpression Lysate (Adult Normal) [NBL1-17152] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GITR.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

GITR/TNFRSF18 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC219997 and based on accession number NM_004195. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens tumor necrosis factor receptor superfamily, member 18 (TNFRSF18), transcript variant 1, mRNA.
Gene
TNFRSF18

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
23.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for GITR/TNFRSF18

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GITR/TNFRSF18 Overexpression Lysate (Native)

  • Activation-inducible TNFR family receptor
  • AITR
  • AITRTNF receptor superfamily activation-inducible protein
  • CD357 antigen
  • CD357
  • GITR
  • GITR-D
  • GITRtumor necrosis factor receptor superfamily member 18
  • Glucocorticoid-induced TNFR-related protein
  • TNFRSF18
  • tumor necrosis factor receptor superfamily, member 18

Background

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longest transcript. It contains an extra coding segment, which leads to a translation frame shift, compared to variant 2. The resulting isoform (1) contains a distinct and shorter C-terminus, as compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for GITR/TNFRSF18 Lysate (NBL1-17152) (0)

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Bioinformatics

Gene Symbol TNFRSF18
Entrez