Recombinant Human GITR Ligand/TNFSF18 Protein

SDS-Page: Human GITR Ligand/TNFSF18 Protein [NBP2-35053]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human GITR Ligand/TNFSF18 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 126 amino acids corresponding to GITR Ligand/TNFSF18 Source: E. coli

  Uniprot ID: Q9UNG2

  Amino Acid Sequence: ETAKEPCMAK FGPLPSKWQM ASSEPPCVNK VSDWKLEILQ NGLYLIYGQV APNANYNDVA PFEVRLYKNK DMIQTLTNKS KIQNVGGTYE LHVGDTIDLI FNSEHQVLKN NTYWGIILLA NPQFIS

• Details of Functionality: GITRL protein is fully biologically active when compared to standard. The biological activity as determined by stimulation of IL-8 production using human PBMC is in a concentration range of 0.1-10 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: TNFSF18
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of GITR Ligand/TNFSF18 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 14.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human GITR Ligand/TNFSF18 Protein

• Activation-inducible TNF-related ligand
• AITRL
• AITRLMGC138237
• GITR Ligand
• GITRL
• GITRLglucocorticoid-induced TNFR-related protein ligand
• Glucocorticoid-induced TNF-related ligand
• hGITRLGITR ligand
• TL6AITR ligand
• TNFSF18
• tumor necrosis factor (ligand) superfamily, member 18
• tumor necrosis factor ligand superfamily member 18

Background

GITRL (AITR Ligand/TNFSF18) is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family and it is encoded by the TNFSF18 gene. This cytokine is a ligand for receptor TNFRSF18/AITR/GITR. It has been shown to modulate T lymphocyte survival in peripheral tissues. It is also found to be expressed in endothelial cells, and is thought to be important for interaction between T lymphocytes and endothelial cells. Recombinant human GITRL (AITR Ligand/TNFSF18) is a 14.3 kDa protein, containing 126 amino acid residues and it shares 56 % and 60 % a.a. sequence identity with murine and rat TNFSF18, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: TNFSF18
• Uniprot:
  • Q9UNG2()
  • Q9UNG2()
  • Q9UNG2()