GITR Ligand/TNFSF18 Overexpression Lysate (Native) Summary
The lysate was created in HEK293T cells, using plasmid ID RC210901 and based on accession number NM_005092. The protein contains a C-terminal DDK tag.
Homo sapiens tumor necrosis factor (ligand) superfamily, member 18 (TNFSF18), mRNA.
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Lysate Details for GITR Ligand/TNFSF18
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for GITR Ligand/TNFSF18 Overexpression Lysate (Native)
- Activation-inducible TNF-related ligand
- GITR Ligand
- GITRLglucocorticoid-induced TNFR-related protein ligand
- Glucocorticoid-induced TNF-related ligand
- hGITRLGITR ligand
- TL6AITR ligand
- tumor necrosis factor (ligand) superfamily, member 18
- tumor necrosis factor ligand superfamily member 18
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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