GITR Ligand/TNFSF18 Antibody (721926) [Alexa Fluor® 700]

• Applications: WB
• Clone: 721926
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 700

GITR Ligand/TNFSF18 Antibody (721926) [Alexa Fluor® 700] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse GITR Ligand/TNFSF18
  Thr47-Ser173
  Accession # NP_899247
• Specificity: Detects mouse GITR Ligand/TNFSF18 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) APRIL, rmBAFF, rmEDA, rmFas Ligand, rmLIGHT, rmOX40 Ligand, rmTNF-alpha , rmTRAIL, rmTRANCE, rmTWEAK, rm4-1BB Ligand, r
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Glucocorticoid-induced TNF receptor superfamily-related protein ligand (GITRL) is a member of the TNF superfamily (TNFSF) and has been designated TNFSF18. Mouse GITRL cDNA encodes a 173 amino acid (aa) type II membrane protein with a C-terminal extracellular domain of 131 aa, an N-terminal cytoplasmic domain of 23 aa and a transmembrane domain of 19 aa. It shares approximately 60% aa sequence identity with human GITRL (2). Mouse GITRL is expressed at high levels in macrophages, dendritic cells and B cells. The expression is transiently upregulated by LPS stimulation. GITRL binds to the type I transmembrane protein GITR/TNFRSF18, which is a member of the TNF receptor superfamily that is predominantly expressed on CD25⁺ regulatory CD4⁺ T cells (Treg). GITR is also expressed on naïve CD4⁺ CD25^- T cells, where its expression is upregulated after antigen-driven activation (1, 2). Ligation of GITR has been found to induce nuclear factor (NF)-kappa B activation via TNF receptor-associated factor 2. GITRL provides costimulatory signals for activated CD4⁺ CD25^- T cells to enhance cell proliferation and augment cytokine production. On CD4⁺ CD25⁺ Treg cells, GITRL also provides costimulatory signals to induce proliferation, setting Treg cells in an active/hyperproliferactive state that reverses the suppressive function of Treg cells. GITRL-GITR ligation provides important costimulatory signals that play important roles in modulating diverse T cell functions (1-4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.