GFR alpha-2/GDNF R alpha-2 Overexpression Lysate

Images

 
Western Blot: GDNF Receptor alpha 2 Overexpression Lysate (Adult Normal) [NBL1-11052] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GDNF Receptor alpha 2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

GFR alpha-2/GDNF R alpha-2 Overexpression Lysate Summary

Description

GFR alpha-2/GDNF R alpha-2 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC208014

Accession#: NM_001495

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
GFRA2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GFR alpha-2/GDNF R alpha-2 Overexpression Lysate

  • GDNF family receptor alpha 2
  • GDNF family receptor alpha-2
  • GDNF R alpha-2
  • GDNF receptor alpha-2
  • GDNF receptor beta
  • GDNFR-alpha-2
  • GDNFR-beta
  • GDNFRBRET ligand 2
  • GFR alpha2
  • GFR alpha-2
  • GFRA2
  • GFRa-2
  • GFR-alpha 2
  • GFR-alpha-2
  • glial cell line derived neurotrophic factor receptor, beta
  • Neurturin receptor alpha
  • NRTNR-alpha
  • NTNRA
  • NTNR-alpha
  • PI-linked cell-surface accessory protein
  • RETL2TGF-beta-related neurotrophic factor receptor 2
  • TGF-beta related neurotrophic factor receptor 2
  • TRN receptor, GPI-anchored
  • TRNR2NRTNR-ALPHA

Background

Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This encoded protein acts preferentially as a receptor for NTN compared to its other family member, GDNF family receptor alpha 1. This gene is a candidate gene for RET-associated diseases. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for GFR alpha-2/GDNF R alpha-2 Lysate (NBL1-11052) (0)

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Bioinformatics

Gene Symbol GFRA2