GFR alpha-2/GDNF R alpha-2 Antibody [Alexa Fluor® 700]

• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 700

GFR alpha-2/GDNF R alpha-2 Antibody [Alexa Fluor® 700] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human GFRα‑2/GDNF Rα‑2
  Ser22-Ser441
  Accession # O00451
• Specificity: Detects human GFR alpha ‑2/GDNF R alpha ‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross‑reactivity with recombinant mouse GFR alpha -2 is observed and less than 5% cross-reactivity with recombinant human GFR alpha -1 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Glial cell line-derived growth factor (GDNF), neurturin (NTN), persephin (PSP) and artemin, distant members of theTGF-beta  superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits (GDNF receptor alpha -1 [GFR alpha -1], also known as Trn R1 or GFR alpha -2, also known as Trn R2). GFR alpha -1 and -2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF or NTN to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase.

Human GFR alpha -2 cDNA encodes a 464 amino acid (aa) residue protein with a putative N-terminal 21 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, human GFR alpha -2 has a C-terminal hydrophobic region which is preceded by a three aa residue (GPS) GPI-binding site. Human GFR alpha -2 shares 96.5% amino acid identity with mouse GFR alpha -2. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.