GFR alpha-2/GDNF R alpha-2 Antibody (71515) [Unconjugated]

GFRa-2/GDNF Ra-2 was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Mouse Anti-Human GFRa-2/ GDNF Ra-2 Monoclonal Antibody (Catalog # MAB6131) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 71515
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

GFR alpha-2/GDNF R alpha-2 Antibody (71515) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human GFR alpha ‑2/GDNF R alpha ‑2
  Ser22-Ser441
  Accession # O00451
• Specificity: Detects human GFR alpha ‑2/GDNF R alpha ‑2 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GFR alpha-2/GDNF R alpha-2 Antibody (71515) [Unconjugated]

• GDNF family receptor alpha 2
• GDNF family receptor alpha-2
• GDNF R alpha-2
• GDNF receptor alpha-2
• GDNF receptor beta
• GDNFR-alpha-2
• GDNFR-beta
• GDNFRBRET ligand 2
• GFR alpha2
• GFR alpha-2
• GFRA2
• GFRa-2
• GFR-alpha 2
• GFR-alpha-2
• glial cell line derived neurotrophic factor receptor, beta
• Neurturin receptor alpha
• NRTNR-alpha
• NTNRA
• NTNR-alpha
• PI-linked cell-surface accessory protein
• RETL2TGF-beta-related neurotrophic factor receptor 2
• TGF-beta related neurotrophic factor receptor 2
• TRN receptor, GPI-anchored
• TRNR2NRTNR-ALPHA

Background

Glial cell line-derived growth factor (GDNF), neurturin (NTN), persephin (PSP) and artemin, distant members of theTGF-beta  superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits (GDNF receptor alpha -1 [GFR alpha -1], also known as GDNF R alpha -1 or Trn R1, or GFR alpha -2, also known as  GDNF R alpha -2 or Trn R2). GFR alpha -1 and -2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF or NTN to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase.

Human GFR alpha -2 cDNA encodes a 464 amino acid (aa) residue protein with a putative N-terminal 21 aa hydrophobic signal peptide. Like other GPI-linked proteins, human GFR alpha -2 has a C-terminal hydrophobic region which is preceded by a three aa (GPS) GPI-binding site. Human GFR alpha -2 shares 96.5% amino acid identity with mouse GFR alpha -2. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.

1. Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
2. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
3. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
4. Baloh, R.H. et al. (1998) Neuron 21:1291.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O00451()