GFR alpha-2/GDNF R alpha-2 Antibody (129030) [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB
• Clone: 129030
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

GFR alpha-2/GDNF R alpha-2 Antibody (129030) [Alexa Fluor® 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human GFRα‑2/GDNF Rα‑2
  Ser22-Ser441
  Accession # O00451
• Specificity: Detects human GFR alpha ‑2/GDNF R alpha ‑2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) GDNF R alpha , rhGFR alpha -3, rhGFRa-4, and recombinant mouse GFR alpha -2 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GFR alpha-2/GDNF R alpha-2 Antibody (129030) [Alexa Fluor® 647]

• GDNF family receptor alpha 2
• GDNF family receptor alpha-2
• GDNF R alpha-2
• GDNF receptor alpha-2
• GDNF receptor beta
• GDNFR-alpha-2
• GDNFR-beta
• GDNFRBRET ligand 2
• GFR alpha2
• GFR alpha-2
• GFRA2
• GFRa-2
• GFR-alpha 2
• GFR-alpha-2
• glial cell line derived neurotrophic factor receptor, beta
• Neurturin receptor alpha
• NRTNR-alpha
• NTNRA
• NTNR-alpha
• PI-linked cell-surface accessory protein
• RETL2TGF-beta-related neurotrophic factor receptor 2
• TGF-beta related neurotrophic factor receptor 2
• TRN receptor, GPI-anchored
• TRNR2NRTNR-ALPHA

Background

Glial cell line-derived growth factor (GDNF), neurturin (NTN), persephin (PSP) and artemin, distant members of theTGF-beta  superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits (GDNF receptor alpha -1 [GFR alpha -1], also known as GDNF R alpha -1 or Trn R1, or GFR alpha -2, also known as  GDNF R alpha -2 or Trn R2). GFR alpha -1 and -2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF or NTN to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase.

Human GFR alpha -2 cDNA encodes a 464 amino acid (aa) residue protein with a putative N-terminal 21 aa hydrophobic signal peptide. Like other GPI-linked proteins, human GFR alpha -2 has a C-terminal hydrophobic region which is preceded by a three aa (GPS) GPI-binding site. Human GFR alpha -2 shares 96.5% amino acid identity with mouse GFR alpha -2. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.