GFI-1 Antibody (5D7)

Images

 
Western Blot: GFI-1 Antibody (5D7) [NBP1-51652] - Western blot analysis using GFI1 mAb against HEK293 (1) and GFI1-hIgGFc transfected HEK293 (2) cell lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
5D7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

GFI-1 Antibody (5D7) Summary

Immunogen
Purified recombinant fragment of human GFI1 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GFI1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for GFI-1 Antibody (5D7)

  • GFI1
  • GFI-1
  • growth factor independence-1
  • growth factor independent 1 transcription repressor
  • growth factor independent 1
  • Growth factor independent protein 1
  • Pal-1
  • SCN2
  • Zinc finger protein 163
  • zinc finger protein Gfi-1
  • ZNF163
  • ZNF163FLJ94509

Background

This gene encodes a nuclear zinc finger protein that functions as a transcriptional repressor. This protein plays a role in diverse developmental contexts, including hematopoiesis and oncogenesis. It functions as part of a complex along with other cofactors to control histone modifications that lead to silencing of the target gene promoters. Mutations in this gene cause autosomal dominant severe congenital neutropenia, and also dominant nonimmune chronic idiopathic neutropenia of adults, which are heterogeneous hematopoietic disorders that cause predispositions to leukemias and infections. Multiple alternatively spliced variants, encoding the same protein, have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GFI1
Entrez