Gemin 5 Antibody - BSA Free

Western Blot: Gemin 5 Antibody [NBP3-12933] - Gemin 5 antibody detects Gemin 5 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Gemin 5 antibody (NBP3-12933) diluted at a dilution of 1:500.

Immunocytochemistry/Immunofluorescence: Gemin 5 Antibody [NBP3-12933] - Gemin 5 antibody detects Gemin 5 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Gemin 5 protein stained by Gemin 5 antibody (NBP3-12933) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: Gemin 5 Antibody [NBP3-12933] - Gemin 5 antibody detects Gemin 5 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Gemin 5 stained by Gemin 5 antibody (NBP3-12933) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Gemin 5 Antibody [NBP3-12933] - Gemin 5 antibody detects Gemin 5 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Gemin 5 stained by Gemin 5 antibody (NBP3-12933) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunoprecipitation: Gemin 5 Antibody [NBP3-12933] - Immunoprecipitation of Gemin 5 protein from HepG2 whole cell extracts using 5 ug of Gemin 5 antibody (NBP3-12933). Western blot analysis was performed using Gemin 5 antibody (NBP3-12933). EasyBlot anti-Rabbit IgG was used as a secondary reagent.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Gemin 5 Antibody - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human Gemin 5. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GEMIN5
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Gemin 5 Antibody - BSA Free

• DKFZp586M1824
• EC 2.7.7.8
• EC 5.4.2.1
• gem (nuclear organelle) associated protein 5
• gem-associated protein 5
• gemin 5
• Gemin5
• MGC142174

Background

GEMIN5 is part of a large macromolecular complex localized to both the cytoplasm and the nucleus that plays a role in the cytoplasmic assembly of small nuclear ribonucleoproteins (snRNPs). Other members of this complex include SMN (MIM 600354), GEMIN2 (SIP1; MIM 602595), GEMIN3 (DDX20; MIM 606168), and GEMIN4 (MIM 606969).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GEMIN5