Gelsolin/GSN Antibody (16H9) - BSA Free

Immunocytochemistry/Immunofluorescence: Gelsolin/GSN Antibody (16H9) [NBP3-26541] - Staining of HepG2 with Gelsolin/GSN Antibody (16H9) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 510-conjugated Goat Anti-Rabbit IgG (H+L).

Flow Cytometry: Gelsolin/GSN Antibody (16H9) [NBP3-26541] - Overlay Peak curve showing HepG2 cells stained with Gelsolin/GSN Antibody (16H9) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF
• Clone: 16H9
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Gelsolin/GSN Antibody (16H9) - BSA Free Summary

• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human Gelsolin/GSN [UniProt P06396]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GSN
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Gelsolin/GSN Antibody (16H9) - BSA Free

• Actin-depolymerizing factor
• ADF
• AGEL
• Brevin
• DKFZp313L0718
• gelsolin (amyloidosis, Finnish type)
• Gelsolin
• GSN

Background

Gelsolin is a calcium regulated actin binding protein. Gelsolin is a regulator of actin filament assembly and disassembly and consists of six homologous subdomains (S1-S6). When C-terminal tail (S6) is bound to calcium ion, it straightens and exposes actin binding sites located on S2s helices, leading to Gelsolin actin-serving activity (1). Gelsolin is inhibited by phosphatidylinositol (4,5)-bisphosphate (PIP2), where PIP2 will bind to S1, S2, and S3 sites. Gelsolin is involved in podosome formation and has been linked to inhibition of apoptosis by preventing release of cytochrome C (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GSN