GEF-H1 Overexpression Lysate (Native)

Images

 
Western Blot: GEF-H1 Overexpression Lysate (Adult Normal) [NBL1-07680] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GEF-H1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

GEF-H1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204546 and based on accession number NM_004723. The protein contains a C-terminal DDK tag.
Epitope
C-terminal Myc/DDK
Specificity
Homo sapiens rho/rac guanine nucleotide exchange factor (GEF) 2 (ARHGEF2), mRNA.
Gene
ARHGEF2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
108.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for GEF-H1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GEF-H1 Overexpression Lysate (Native)

  • GEF
  • GEFH1
  • GEF-H1DKFZp547P1516
  • Guanine nucleotide exchange factor H1
  • KIAA0651DKFZp547L106
  • LFP40FLJ45440
  • Microtubule-regulated Rho-GEF
  • P40
  • Proliferating cell nucleolar antigen p40
  • rho guanine nucleotide exchange factor 2
  • Rho/Rac guanine nucleotide exchange factor (GEF) 2
  • Rho/Rac guanine nucleotide exchange factor 2

Background

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for GEF-H1 Lysate (NBL1-07680) (0)

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Bioinformatics

Gene Symbol ARHGEF2