GDNF Antibody (27165) [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 27165
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

GDNF Antibody (27165) [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human GDNF
  Arg109-Ile211
  Accession # P39905
• Specificity: Detects human GDNF in ELISAs
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GDNF Antibody (27165) [Alexa Fluor® 700]

• Astrocyte-derived trophic factor
• ATF
• ATF1
• ATF2
• GDNF
• glial cell derived neurotrophic factor
• glial cell line derived neurotrophic factor
• glial cell line-derived neurotrophic factor
• HFB1-GDNF
• HGDNF
• HSCR3

Background

Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons. Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF‑beta  superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF‑beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell‑expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.