GCLC Antibody

Product Discontinued

GCLC Antibody Summary

• Immunogen: Synthetic peptide corresponding to internal region of rat heavy subunit of GCS protein.
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GCLC
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
  • Western Blot 1:100-1:2000
• Application Notes: For IHC-P Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
• Theoretical MW: 73 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24467268)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.6) and 1.0% BSA
• Preservative: 0.1% Sodium Azide
• Purity: Immunogen affinity purified

Notes

For a positive control use Breast carcinoma.

Alternate Names for GCLC Antibody

• EC 6.3.2.2
• Gamma-ECS
• Gamma-glutamylcysteine synthetase
• GCLC
• GCS heavy chain
• GCS
• GLCL
• GLCLC
• GLCLCGCL
• glutamate--cysteine ligase catalytic subunit
• glutamate-cysteine ligase, catalytic subunit

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GCLC Antibody (NBP1-49762)(9)

Publications using NBP1-49762

• Lee JA, Kim DJ KMS99220 Exerts Anti-Inflammatory Effects, Activates the Nrf2 Signaling and Interferes with IKK, JNK and p38 MAPK via HO-1 Mol. Cells 2019-10-31 [PMID: 31656063] (WB, Mouse)
• Hancock M, Hafstad AD, Nabeebaccus AA et al. Myocardial NADPH oxidase-4 regulates the physiological response to acute exercise Elife 2018-12-27 [PMID: 30589411] (WB, Mouse)
• Park SS, Lee DM, Lim JH et al. Pyrrolidine dithiocarbamate reverses Bcl-xL-mediated apoptotic resistance to doxorubicin by inducing paraptosis Carcinogenesis 2018-01-10 [PMID: 29329420] (Human)
• Lee JA, Son HJ, Kim JH et al. A novel synthetic isothiocyanate ITC-57 displays antioxidant, anti-inflammatory, and neuroprotective properties in a mouse Parkinson's disease model. Free Radic. Res. 2016-09-06 [PMID: 27598306] (WB, Mouse)
• Park JH, Choi JW, Ju EJ et al. Antioxidant and Anti-Inflammatory Activities of a Natural Compound, Shizukahenriol, through Nrf2 Activation. Molecules 2015-09-14 [PMID: 26364630] (WB, Mouse)
• Lee JA, Son HJ, Park KD et al. A Novel Compound ITC-3 Activates the Nrf2 Signaling and Provides Neuroprotection in Parkinson's Disease Models. Neurotox Res. 2015-08-02 [PMID: 26233727]
• Woo SY, Kim JH, Moon MK et al. Discovery of Vinyl Sulfones as a Novel Class of Neuroprotective Agents toward Parkinson's Disease Therapy. J. Med. Chem. 2014-02-27 [PMID: 24467268] (WB, Mouse)
• Choi JW, Kim GJ, Kim HJ et al. Identification and evaluation of a napyradiomycin as a potent Nrf2 activator: Anti-oxidative and anti-inflammatory activities Bioorg Chem 2020-10-27 [PMID: 33161250] (WB, Mouse)
  
  Details:
  Western blot analysis of BV-2 cells treated with extracts from Streptomyces sp. Strain YP127.

FAQs for GCLC Antibody (NBP1-49762). (Showing 1 - 1 of 1 FAQs).

1. Could you let me know the dilution factor of NBP1-49762 for Western Blot?
   • The recommended starting dilution for Western Blot is 1:1000-1:2000. This of course is only a recommended starting dilution so depending on the samples used, the dilution should be optimized accordingly.

Bioinformatics

• Gene Symbol: GCLC
• Entrez:
  • 2729(Human)
  • 25283(Rat)
• Uniprot:
  • P19468(Rat)