Please see the vial label for concentration. If unlisted please contact technical services.
Immunogen affinity purified
For IHC-P Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NBP1-49762 in the following applications:
Mouse reactivity reported in scientific literature (PMID: 24467268)
For a positive control use Breast carcinoma.
Alternate Names for GCLC Antibody
GCS heavy chain
glutamate--cysteine ligase catalytic subunit
glutamate-cysteine ligase, catalytic subunit
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for GCLC Antibody (NBP1-49762). (Showing 1 - 1 of 1 FAQs).
Could you let me know the dilution factor of NBP1-49762 for Western Blot?
The recommended starting dilution for Western Blot is 1:1000-1:2000. This of course is only a recommended starting dilution so depending on the samples used, the dilution should be optimized accordingly.