GCLC Antibody (JA08-03)

Western Blot: GCLC Antibody (JA08-03) [NBP2-66864] - Analysis of GCLC on A431 cell using anti-GCLC antibody at 1/1,000 dilution.

Immunohistochemistry-Paraffin: GCLC Antibody (JA08-03) [NBP2-66864] - Analysis of paraffin-embedded rat kidney tissue using anti-GCLC antibody. Counter stained with hematoxylin.

Flow Cytometry: GCLC Antibody (JA08-03) [NBP2-66864] - Analysis of Jurkat cells with GCLC antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Immunohistochemistry-Paraffin: GCLC Antibody (JA08-03) [NBP2-66864] - Analysis of paraffin-embedded human fetal kidney tissue using anti-GCLC antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: GCLC Antibody (JA08-03) [NBP2-66864] - Analysis of paraffin-embedded human fetal liver tissue using anti-GCLC antibody. Counter stained with hematoxylin.

Product Discontinued

GCLC Antibody (JA08-03) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human GCLC aa 588-637 / 637. (SwissProt: P48506 Human; SwissProt: P19468 Rat)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GCLC
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for GCLC Antibody (JA08-03)

• EC 6.3.2.2
• Gamma-ECS
• Gamma-glutamylcysteine synthetase
• GCLC
• GCS heavy chain
• GCS
• GLCL
• GLCLC
• GLCLCGCL
• glutamate--cysteine ligase catalytic subunit
• glutamate-cysteine ligase, catalytic subunit

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GCLC