| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4004-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03451-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38491-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98484-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03450-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98484-100ul | |||
| BSA Free | NBP2-98484-50ul | |||
![Western Blot: GBL Antibody [NBP3-03451] - Western blot analysis of extracts of various cell lines, using GBL Rabbit pAb (NBP3-03451) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/GBL Antibody-Western Blot-NBP3-03451-img0006.jpg "Western Blot: GBL Antibody [NBP3-03451] - Western blot analysis of extracts of various cell lines, using GBL Rabbit pAb (NBP3-03451) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunocytochemistry/Immunofluorescence: GBL Antibody [NBP3-03451] - Analysis of U2OS cells using GBL antibody .](http://images.novusbio.com/fullsize/GBL-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03451-img0001.jpg)
![Immunohistochemistry-Paraffin: GBL Antibody [NBP3-03451] - Immunohistochemistry of paraffin-embedded rat kidney using GBL Rabbit pAb (NBP3-03451) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/GBL-Antibody-Immunohistochemistry-Paraffin-NBP3-03451-img0005.jpg)
![Immunohistochemistry-Paraffin: GBL Antibody [NBP3-03451] - Immunohistochemistry of paraffin-embedded human liver using GBL Rabbit pAb (NBP3-03451) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/GBL-Antibody-Immunohistochemistry-Paraffin-NBP3-03451-img0002.jpg)
![Immunohistochemistry-Paraffin: GBL Antibody [NBP3-03451] - Immunohistochemistry of paraffin-embedded human lung cancer using GBL Rabbit pAb (NBP3-03451) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/GBL-Antibody-Immunohistochemistry-Paraffin-NBP3-03451-img0003.jpg)
![Immunohistochemistry-Paraffin: GBL Antibody [NBP3-03451] - Immunohistochemistry of paraffin-embedded mouse stomach using GBL Rabbit pAb (NBP3-03451) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/GBL-Antibody-Immunohistochemistry-Paraffin-NBP3-03451-img0004.jpg)
![Western Blot: GBL Antibody [NBP2-38491] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HEK 293](http://images.novusbio.com/fullsize/GBL-Antibody-Western-Blot-NBP2-38491-img0003.jpg "Western Blot: GBL Antibody [NBP2-38491] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HEK 293")
![Immunocytochemistry/Immunofluorescence: GBL Antibody [NBP2-38491] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/GBL-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38491-img0004.jpg)
![Immunohistochemistry: GBL Antibody [NBP2-38491] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/GBL-Antibody-Immunohistochemistry-NBP2-38491-img0002.jpg)
![Western Blot: GBL Antibody [NBP2-98484] - Anti-GBL rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 38 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/GBL-Antibody-Western-Blot-NBP2-98484-img0004.jpg "Western Blot: GBL Antibody [NBP2-98484] - Anti-GBL rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 38 kDa (We are unsure as to the identity of these extra bands).")
![Immunohistochemistry-Paraffin: GBL Antibody [NBP2-98484] - Immunochemical staining of human GBL in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/GBL-Antibody-Immunohistochemistry-Paraffin-NBP2-98484-img0003.jpg)
![Immunoprecipitation: GBL Antibody [NBP2-98484] - GBL was immunoprecipitated using: Lane A: 0.5 mg U-251 MG Whole Cell Lysate 4 ul anti-GBL rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-GBL rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 35 kDa](http://images.novusbio.com/fullsize/GBL-Antibody-Immunoprecipitation-NBP2-98484-img0005.jpg)
![Western Blot: GBL Antibody [NBP3-03450] - Analysis of extracts of various cell lines, using GBL antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/GBL-Antibody-Western-Blot-NBP3-03450-img0002.jpg "Western Blot: GBL Antibody [NBP3-03450] - Analysis of extracts of various cell lines, using GBL antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![<b>Genetic Strategies Validation. </b>Western Blot: GBL Antibody - BSA Free [NBP3-03450] - Western blot analysis of extracts from wild type(WT) and GBL knockdown (KD) 293T cells, using GBL Rabbit pAb (A1059) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-03450_rabbit-polyclonal-gbl-antibody-52202519313474.jpg)
![Western Blot: GBL Antibody - BSA Free [NBP3-03450] - Western blot analysis of various lysates, using GBL Rabbit pAb (A1059) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-03450_rabbit-polyclonal-gbl-antibody-52202519313450.jpg)
![Immunoprecipitation: GBL Antibody [NB100-2124] - Detection of human GbetaL by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GbetaL antibody NB100-2124 used for IP at 6 ug per reaction. GbetaL was also immunoprecipitated by another rabbit anti-GbetaL antibody. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/GBL-Antibody-NB100-2124-img0006.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: GBL Antibody [NB100-2124] - Detection of human GbetaL by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GbetaL antibody NB100-2124 used for IP at 6 ug per reaction. GbetaL was also immunoprecipitated by another rabbit anti-GbetaL antibody. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: G protein beta subunit like Overexpression Lysate (Adult Normal) [NBL1-10995] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for G protein beta subunit like.](http://images.novusbio.com/fullsize/G-protein-beta-subunit-like-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10995-img0002.jpg "Western Blot: G protein beta subunit like Overexpression Lysate (Adult Normal) [NBL1-10995] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for G protein beta subunit like.")