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GATE-16/GABARAPL2 Antibody (853746) [Unconjugated]

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Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, mouse brain (cerebellum) tissue, and rat brain (total) tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse/Rat GATE-16 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
853746
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

GATE-16/GABARAPL2 Antibody (853746) [Unconjugated] Summary

Immunogen
E.coli-derived recombinant human GATE16
Accession # P60520
Specificity
Detects human, mouse, and rat GATE-16 in Western blot.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
GABARAPL2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GATE-16/GABARAPL2 Antibody (853746) [Unconjugated]

  • Apg8p2
  • ATG8
  • ATG8C
  • FLC3A
  • GABARAPL2
  • gamma-aminobutyric acid receptor-associated protein-like 2
  • Ganglioside expression factor 2
  • GATE16
  • GATE-16
  • GEF2
  • GEF-2
  • GEF2GEF-2
  • General Protein Transport Factor P16
  • Golgi-Associated ATPase Enhancer Of 16 KDa
  • MAP1 light chain 3 related protein
  • MAP1 light chain 3-related protein

Background

Golgi-associated ATPase Enhancer of 16 kDa (GATE-16), also known as Apg8p2 and GABARAPL2, is a 117 amino acid (aa) polypeptide and a member of the Autophagy-related 8 (Atg8) family of proteins (1). GATE-16/Apg8p2 has 100% aa sequence identity with its mouse and rat orthologs, and is orthologous to the yeast Atg8. Atg8 family members show structural similarity with Ubiquitin, but lack aa sequence similarity. GATE-16/Apg8p2 is best known for its role in autophagy (2,3). GATE-16/Apg8p2 covalently attaches to phosphatidylethanolamine (PE) the phagophore (autophagosome precursor) membrane using a Ubiquitin-like conjugation system that includes Ubiquitin-activating (E1)-, Ubiquitin-conjugating (E2)-, and Ubiquitin Ligase (E3)-like enzymes. Here it is involved in the later stages of autophagosome formation (4,5). It may also be involved in cargo recruitment to autophagosomes (1).

  1. Shpilka, T. et al. (2011) Genome Biol. 12:226.
  2. Wang, H. et al. (1999) Nature 397:69.
  3. Leil, T.A. et al. (2004) J. Neurosci. 24:11429.
  4. Weidberg, H. et al. (2010) EMBO J. 29:1792.
  5. Weidberg, H. et al. (2011) Ann. Rev. Biochem. 80:125

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GABARAPL2
Uniprot