GATE-16/GABARAPL2 Antibody (853746) [Alexa Fluor® 750]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 853746
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 750

GATE-16/GABARAPL2 Antibody (853746) [Alexa Fluor® 750] Summary

• Immunogen: E.coli-derived recombinant human GATE16
  Accession # P60520
• Specificity: Detects human, mouse, and rat GATE-16 in Western blot.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GATE-16/GABARAPL2 Antibody (853746) [Alexa Fluor® 750]

• Apg8p2
• ATG8
• ATG8C
• FLC3A
• GABARAPL2
• gamma-aminobutyric acid receptor-associated protein-like 2
• Ganglioside expression factor 2
• GATE16
• GATE-16
• GEF2
• GEF-2
• GEF2GEF-2
• General Protein Transport Factor P16
• Golgi-Associated ATPase Enhancer Of 16 KDa
• MAP1 light chain 3 related protein
• MAP1 light chain 3-related protein

Background

Golgi-associated ATPase Enhancer of 16 kDa (GATE-16), also known as Apg8p2 and GABARAPL2, is a 117 amino acid (aa) polypeptide and a member of the Autophagy-related 8 (Atg8) family of proteins (1). GATE-16/Apg8p2 has 100% aa sequence identity with its mouse and rat orthologs, and is orthologous to the yeast Atg8. Atg8 family members show structural similarity with Ubiquitin, but lack aa sequence similarity. GATE-16/Apg8p2 is best known for its role in autophagy (2,3). GATE-16/Apg8p2 covalently attaches to phosphatidylethanolamine (PE) the phagophore (autophagosome precursor) membrane using a Ubiquitin-like conjugation system that includes Ubiquitin-activating (E1)-, Ubiquitin-conjugating (E2)-, and Ubiquitin Ligase (E3)-like enzymes. Here it is involved in the later stages of autophagosome formation (4,5). It may also be involved in cargo recruitment to autophagosomes (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for GATE-16/GABARAPL2 Antibody (FAB9035S). (Showing 1 - 1 of 1 FAQ).

1. I am currently interested in detecting GABARAPL2 by immunofluorescence. Do you have an antibody that I could try out for immunofluorescence
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