GART Antibody (SC66-02)

Western Blot: GART Antibody (SC66-02) [NBP2-67612] - Analysis of GART on different lysates using anti-GART antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293T Lane 3: A431

Immunocytochemistry/Immunofluorescence: GART Antibody (SC66-02) [NBP2-67612] - Staining GART in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: GART Antibody (SC66-02) [NBP2-67612] - Analysis of paraffin-embedded mouse uterus tissue using anti-GART antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: GART Antibody (SC66-02) [NBP2-67612] - Staining GART in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: GART Antibody (SC66-02) [NBP2-67612] - Staining GART in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: GART Antibody (SC66-02) [NBP2-67612] - Analysis of paraffin-embedded human tonsil tissue using anti-GART antibody. Counter stained with hematoxylin.

Product Discontinued

GART Antibody (SC66-02) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human GART aa 570-750 / 1010. (SwissProt: P22102 Human; SwissProt: Q64737 Mouse; Unigene: 55532 Rat)
• Localization: Cytoplasm, Nucleus, Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GART
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for GART Antibody (SC66-02)

• AIRS
• EC 6.3.3.1
• EC 6.3.4.13
• GARS
• GARTF
• MGC47764
• PGFTEC 2.1.2.210PAIS
• phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamidesynthetase, phosphoribosylaminoimidazole synthetase
• trifunctional purine biosynthetic protein adenosine-3

Background

GART is encoded by this gene is a trifunctional polypeptide. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. This enzyme is highly conserved in vertebrates. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GART