gamma H2AX [p Ser139] Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: gamma H2AX [p Ser139] Antibody [NB100-384] - Samples: Neocarzinostatin treated asynchronous HeLa cells (left) and untreated asynchronous HeLa cells (right). Antibody: Affinity ...read more
Flow Cytometry: gamma H2AX [p Ser139] Antibody [NB100-384] - Analysis of gamma-H2AX in Etoposide Treated Jurkat Cells. Cells were treated for 3 hrs in 5ug/ml etoposide, fixed in 1.5% PFA, and permeabilized in 90% ...read more
Biological Strategies: Western Blot: gamma H2AX [p Ser139] Antibody [NB100-384] - Samples: Nuclear extract from HeLa cells treated with 100 uM etoposide for 4 hours (+) or mock treated (-). Antibody: Affinity ...read more
Immunohistochemistry: gamma H2AX [p Ser139] Antibody [NB100-384] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti- gamma-H2AX used at a dilution of 1:1,000 (1 ug/ml). ...read more
Immunohistochemistry: gamma H2AX [p Ser139] Antibody [NB100-384] - Samples: Neocarzinostatin treated asynchronous HeLa cells (left) and untreated asynchronous HeLa cells (right). Antibody: Affinity purified rabbit ...read more
Biological Strategies: Western Blot: gamma H2AX [p Ser139] Antibody [NB100-384] - Detection of Human and Mouse gamma H2AX by Western Blot. Samples: Nuclear extract (50 ug) from human HEK293, human melanoma ...read more
Simple Western: gamma H2AX [p Ser139] Antibody [NB100-384] - Simple Western lane view shows a specific band for gamma H2AX in 0.2 mg/ml of Jurkat lysate(s). This experiment was performed under reducing conditions using ...read more
Simple Western: gamma H2AX [p Ser139] Antibody [NB100-384] - Electropherogram image(s) of corresponding Simple Western lane view. Gamma H2AX antibody was used at 5 ug/ml dilution on Jurkat lysate(s).

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

gamma H2AX [p Ser139] Antibody Summary

Immunogen
This gamma H2AX antibody maps to a region surrounding phosphorylated serine 139 of human histone H2AX [Swiss-Prot entry P16104] (GeneID 3014).
Modification
p Ser139
Localization
Nucleous, chromosome.
Marker
DNA Double-strand break marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
H2AFX
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • Simple Western 5 ug/ml
  • Flow Cytometry 5 ug per 1 million cells
  • Immunocytochemistry/Immunofluorescence 1:500 - 1:5000
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Frozen 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
Application Notes
This antibody is useful for: Western blot, ICC, IHC and FLOW Cytometry. For IHC, epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehydefixed cells. Immunoprecipitation is not recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
gamma H2AX Lysate (NBL1-11424)
Reviewed Applications
Read 1 Review rated 5
using
NB100-384 in the following applications:

Publications
Read Publications using
NB100-384 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27102221), Canine reactivity reported in scientific literature (PMID: 23365434).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

Licensed to Novus Biologicals LLC under U.S. Patent Nos. 6,362,317 and 6,884,873.

Alternate Names for gamma H2AX [p Ser139] Antibody

  • H2A histone family, member X
  • H2A.X
  • H2a/x
  • H2AX histone
  • H2AXhistone H2A.x
  • YH2AX

Background

Histone H2AX is a 14 kDa ubiquitous member of the H2A histone family. It contains an evolutionary conserved SQ motif at the C-terminus in eukaryotes. Serine 139 within this motif becomes rapidly phosphorylated to yield a form known as gamma-H2AX in response to double-strand DNA damage. The response of eukaryotic cells to double-strand breaks in genomic DNA includes the sequestration of many factors into nuclear foci. H2AX becomes extensively phosphorylated within 1-3 minutes of DNA damage and forms foci at break sites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for gamma H2AX Antibody (NB100-384)(67)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.

We have publications tested in 7 applications: FLOW, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
FLOW
(2)
Flow
(1)
ICC/IF
(19)
IHC
(4)
IHC-Fr
(1)
IHC-P
(1)
WB
(8)
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Human
(24)
Mouse
(10)
Rat
(1)
Canine
(1)
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Showing Publications 1 - 10 of 67. Show All 67 Publications.
Publications using NB100-384 Applications Species
Philion C, Ma D, Ruvinov I et al. Cymbopogon citratus and Camellia sinensis extracts selectively induce apoptosis in cancer cells and reduce growth of lymphoma xenografts in vivo. Oncotarget 2017 Nov 18 [PMID: 29340014] (IHC-Fr, Human) IHC-Fr Human
Affronti HC, Long MD, Rosario SR et al. Dietary folate levels alter the kinetics and molecular mechanism of prostate cancer recurrence in the CWR22 model. Oncotarget 2017 Nov 28 [PMID: 29262598] (WB, Mouse) WB Mouse
Long J, Huang C, Chen Y et al. Telomeric TERB1-TRF1 interaction is crucial for male meiosis Nat. Struct. Mol. Biol. 2017 Oct 30 [PMID: 29083416] (Mouse) Mouse
Aguilera KY, Huang H, Du W et al. Inhibition of discoidin domain receptor 1 reduces collagen-mediated tumorigenicity in pancreatic ductal adenocarcinoma Mol. Cancer Ther. 2017 Sep 01 [PMID: 28864681] (Human) Human
Valtcheva N, Lang FM, Noske A et al. Tracking the origin of simultaneous endometrial and ovarian cancer by next-generation sequencing - a case report. BMC Cancer Jan 19 2017 [PMID: 28103826] (IHC, Human) IHC Human
Oe S, Miyagawa K, Honma Y, Harada M. Copper induces hepatocyte injury due to the endoplasmic reticulum stress in cultured cells and patients with Wilson disease. Exp. Cell Res. Aug 5 2016 [PMID: 27502587]
Mata-Garrido J, Casafont I, Tapia O et al. Neuronal accumulation of unrepaired DNA in a novel specific chromatin domain: structural, molecular and transcriptional characterization. Acta Neuropathol Commun. 2016 Apr 22 [PMID: 27102221] (ICC/IF, Rat) ICC/IF Rat
Dahlhoff M, Camera E, Schafer M et al. Sebaceous lipids are essential for water repulsion, protection against UVB-induced apoptosis, and ocular integrity in mice. Development. 2016 Mar 17 [PMID: 26989175] (IHC-P, Mouse) IHC-P Mouse
Chen L, Peng Z, Meng Q et al. Loss of IkB kinase beta promotes myofibroblast transformation and senescence through activation of the ROS-TGFbeta autocrine loop Protein Cell Mar 5 2016 [PMID: 26946493] (WB, Mouse) WB Mouse
Francia S, Cabrini M, Matti V et al. DICER, DROSHA and DNA damage-response RNAs are necessary for the secondary recruitment of DNA damage response factors J. Cell. Sci. Feb 16 2016 [PMID: 26906421] (ICC/IF, Human) ICC/IF Human
Show All 67 Publications.

Review for gamma H2AX Antibody (NB100-384) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-384:
Filter by Applications
IF
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IF Human 10/04/2013
View

Summary

ApplicationImmunofluorescence
SpeciesHuman
Pub Med ID24013174
FileView PDF

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for gamma H2AX Antibody (NB100-384). (Showing 1 - 1 of 1 FAQs).

  1. Can we apply antibody gamma H2AX [p Ser139] Antibody (NB100-384) (0.1 ml) for drosophila?
    • NB100-384 has been validated for use in human and mouse, it has not been tested nor validated for use in Drosophila. If you would be interested in testing this novel species, please take a look at our Innovators Reward Program.

Positive Control Lysate(s)

Secondary Antibodies

 

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Blogs on gamma H2AX.

Further unraveling the role of gamma H2AX in DNA damage response
Our genome experiences a moderate amount of DNA damage in our cells on a daily basis.  This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry.  While normal rates of DNA ...  Read full blog post.

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Recent Reviews

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Anonymous
10/04/2013
Application: IF
Species: Human

Bioinformatics

Gene Symbol H2AFX
Entrez
Uniprot