gamma H2AX [p Ser139] Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: gamma H2AX [p Ser139] Antibody [NB100-384] - Samples: Neocarzinostatin treated asynchronous HeLa cells (left) and untreated asynchronous HeLa cells (right). Antibody: Affinity ...read more
Flow Cytometry: gamma H2AX [p Ser139] Antibody [NB100-384] - Analysis of gamma-H2AX in EPE Treated Jurkat Cells. Cells were treated for 3 hrs in 5ug/ml etoposide, fixed in 1.5% PFA, and permeabilized in 90% Methanol. 1 ...read more
Biological Strategies: Western Blot: gamma H2AX [p Ser139] Antibody [NB100-384] - Samples: Nuclear extract from HeLa cells treated with 100 uM EPE for 4 hours (+) or mock treated (-). Antibody: Affinity purified ...read more
Immunohistochemistry: gamma H2AX [p Ser139] Antibody [NB100-384] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti- gamma-H2AX used at a dilution of 1:1,000 (1 ug/ml). ...read more
Immunohistochemistry: gamma H2AX [p Ser139] Antibody [NB100-384] - Samples: Neocarzinostatin treated asynchronous HeLa cells (left) and untreated asynchronous HeLa cells (right). Antibody: Affinity purified rabbit ...read more
Western Blot: gamma H2AX [p Ser139] Antibody [NB100-384] - Detection of human gamma-H2AX by western blot. Samples: Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM EPE for 4 hours (+) or mock treated (-). ...read more
Immunohistochemistry: gamma H2AX [p Ser139] Antibody [NB100-384] - Detection of mouse gamma-H2AX by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- ...read more
Genetic Strategies: Western Blot: gamma H2AX [p Ser139] Antibody [NB100-384] - Detection of Human and Mouse gamma H2AX by Western Blot. Samples: Nuclear extract (50 ug) from human HEK293, human melanoma (G361), ...read more
Immunohistochemistry: gamma H2AX [p Ser139] Antibody [NB100-384] - Detection of human gamma-H2AX by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- ...read more
Simple Western: gamma H2AX [p Ser139] Antibody [NB100-384] - Simple Western lane view shows a specific band for gamma H2AX in 0.2 mg/ml of Jurkat lysate(s). This experiment was performed under reducing conditions using ...read more
Simple Western: gamma H2AX [p Ser139] Antibody [NB100-384] - Electropherogram image(s) of corresponding Simple Western lane view. Gamma H2AX antibody was used at 5 ug/ml dilution on Jurkat lysate(s).

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, ChIP, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

gamma H2AX [p Ser139] Antibody Summary

Immunogen
This gamma H2AX antibody maps to a region surrounding phosphorylated serine 139 of human histone H2AX [Swiss-Prot entry P16104] (GeneID 3014).
Modification
p Ser139
Localization
Nucleous, chromosome.
Marker
DNA Double-strand break marker
Specificity
The epitope maps to a region surrounding phosphorylated serine 139 of human histone H2AX.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
H2AX
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:25000
  • Simple Western 5 ug/ml
  • Flow Cytometry 5 ug per 1 million cells
  • Immunocytochemistry/Immunofluorescence 1:500 to 1:5000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 to 1:5000
  • Chromatin Immunoprecipitation (ChIP)
  • Knockout Validated
Application Notes
For IHC, epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehydefixed cells. Immunoprecipitation is not recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 30049290).
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Histone H2AX Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-384 in the following applications:

Publications
Read Publications using
NB100-384 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27102221), Canine reactivity reported in scientific literature (PMID: 23365434).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

Licensed to Novus Biologicals LLC under U.S. Patent Nos. 6,362,317 and 6,884,873.

Alternate Names for gamma H2AX [p Ser139] Antibody

  • H2A histone family, member X
  • H2A.X
  • H2a/x
  • H2AX histone
  • H2AXhistone H2A.x
  • YH2AX

Background

Histone H2AX is a 14 kDa ubiquitous member of the H2A histone family. It contains an evolutionary conserved SQ motif at the C-terminus in eukaryotes. Serine 139 within this motif becomes rapidly phosphorylated to yield a form known as gamma-H2AX in response to double-strand DNA damage. The response of eukaryotic cells to double-strand breaks in genomic DNA includes the sequestration of many factors into nuclear foci. H2AX becomes extensively phosphorylated within 1-3 minutes of DNA damage and forms foci at break sites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for gamma H2AX Antibody (NB100-384)(90)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Canine, Human .

We have publications tested in 8 applications: ChIP, FLOW, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ChIP
(1)
FLOW
(2)
Flow
(3)
ICC/IF
(26)
IHC
(6)
IHC-Fr
(1)
IHC-P
(3)
WB
(15)
All Applications
Filter By Species
Human
(33)
Mouse
(18)
Rat
(3)
Canine
(2)
Human
(1)
All Species
Showing Publications 1 - 10 of 90. Show All 90 Publications.
Publications using NB100-384 Applications Species
Boye P, Floch F, Serres F et al. Randomized, double-blind trial of F14512, a polyamine-vectorized anticancer drug, compared with etoposide phosphate, in dogs with naturally occurring lymphoma Oncotarget Feb 18 2020 [PMID: 32133044] (Flow, Canine) Flow Canine
Hayashi K, Hishikawa A, Hashiguchi A et al. Association of glomerular DNA damage and DNA methylation with one-year eGFR decline in IgA nephropathy Sci Rep Jan 14 2020 [PMID: 31937846] (IHC-P, Human) IHC-P Human
Quach C, Song Y, Guo H, et al. A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice Nat Commun Dec 12 2019 [PMID: 31831743] (WB, Mouse) WB Mouse
Pan F, Chen Y, He JZ, et al. Dietary riboflavin deficiency promotes N-nitrosomethylbenzylamine-induced esophageal tumorigenesis in rats by inducing chronic inflammation Am J Cancer Res Nov 1 2019 [PMID: 31815047] (IHC, Rat) IHC Rat
Krieger KL, Hu WF, Ripperger T, Woods NT Functional Impacts of the BRCA1-mTORC2 Interaction in Breast Cancer Int J Mol Sci Nov 23 2019 [PMID: 31771139] (WB, Human) WB Human
Kim JJ, Lee SY, Gong F et al. Systematic bromodomain protein screens identify homologous recombination and R-loop suppression pathways involved in genome integrity Genes Dev. Dec 1 2019 [PMID: 31753913] (Human) Human
Lee YY, Cho YJ, Shin SW et al. Anti-Tumor Effects of Wee1 Kinase Inhibitor with Radiotherapy in Human Cervical Cancer Sci Rep 2019 Oct 28 [PMID: 31659268] (IHC-P, Human) IHC-P Human
Haeger A, Alexander S, Vullings M et al. Collective cancer invasion forms an integrin-dependent radioresistant niche J. Exp. Med. Jan 6 2020 [PMID: 31658985] (WB) WB
Ye S, Ding YF, Jia WH et al. SET domain-containing protein 4 epigenetically controls breast cancer stem cell quiescence Cancer Res. Jul 15 2019 [PMID: 31308046] (ICC/IF, Human) ICC/IF Human
Hu WF, Krieger KL, Lagundzin D et al. CTDP1 regulates breast cancer survival and DNA repair through BRCT-specific interactions with FANCI Cell Death Discov Jun 19 2019 [PMID: 31240132] (WB, Human) WB Human
Show All 90 Publications.

Review for gamma H2AX Antibody (NB100-384) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-384:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IF Human 10/04/2013
View

Summary

ApplicationImmunofluorescence
SpeciesHuman
Pub Med ID24013174
FileView PDF

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for gamma H2AX Antibody (NB100-384). (Showing 1 - 1 of 1 FAQs).

  1. Can we apply antibody gamma H2AX [p Ser139] Antibody (NB100-384) (0.1 ml) for drosophila?
    • NB100-384 has been validated for use in human and mouse, it has not been tested nor validated for use in Drosophila. If you would be interested in testing this novel species, please take a look at our Innovators Reward Program.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-384AF350
Alexa Fluor 405 NB100-384AF405
Alexa Fluor 488 NB100-384AF488
Alexa Fluor 532 NB100-384AF532
Alexa Fluor 594 NB100-384AF594
Alexa Fluor 647 NB100-384AF647
Alexa Fluor 700 NB100-384AF700
Alexa Fluor 750 NB100-384AF750
Allophycocyanin NB100-384APC
Biotin NB100-384B
DyLight 350 NB100-384UV
DyLight 405 NB100-384V
DyLight 488 NB100-384G
DyLight 550 NB100-384R
DyLight 594 NB100-384DL594
DyLight 650 NB100-384C
DyLight 680 NB100-384FR
DyLight 755 NB100-384IR
FITC NB100-384F
HRP NB100-384H
Janelia Fluor 549 NB100-384JF549
Janelia Fluor 646 NB100-384JF646
PE NB100-384PE
PerCP NB100-384PCP

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Blogs on gamma H2AX.

Further unraveling the role of gamma H2AX in DNA damage response
Our genome experiences a moderate amount of DNA damage in our cells on a daily basis.  This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry.  While normal rates of DNA ...  Read full blog post.

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Recent Reviews

5
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1
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1
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Anonymous
10/04/2013
Application: IF
Species: Human

Bioinformatics

Gene Symbol H2AX
Entrez
Uniprot