Histone H2AX [p Ser139] Antibody [Alexa Fluor™ Plus 647]

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, Flow, ICC/IF, IHC, ChIP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 647

Histone H2AX [p Ser139] Antibody [Alexa Fluor™ Plus 647] Summary

• Immunogen: This Histone H2AX [p Ser139] Antibody was developed against to a region surrounding phosphorylated serine 139 of human histone H2AX [Swiss-Prot entry P16104] (GeneID 3014).
• Modification: p Ser139
• Localization: Nucleous, chromosome.
• Marker: DNA Double-strand break marker
• Specificity: The epitope maps to a region surrounding phosphorylated serine 139 of human histone H2AX.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: H2AX
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Knockout Validated
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27102221), Canine reactivity reported in scientific literature (PMID: 23365434).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Histone H2AX [p Ser139] Antibody [Alexa Fluor™ Plus 647]

• H2A.X
• H2A/X
• H2AFX
• Histone H2AX

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H2AX