gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human gamma-Glutamylcyclotransferase/CRF21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5086) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for gamma-Glutamylcyclotransferase/CRF21 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody Summary

• Immunogen: E. coli-derived recombinant human gamma ‑Glutamylcyclotransferase/CRF21
  Met1-Leu188
  Accession # O75223
• Specificity: Detects human gamma ‑Glutamylcyclotransferase/CRF21 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: GGCT
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody

• C7orf24
• CRF21
• gammaGlutamylcyclotransferase
• gamma-Glutamylcyclotransferase
• GCTG
• GGC
• GGCT
• LOC79017
• MGC3077

Background

Gamma-glutamylcyclotransferase  (GGCT; also Cytochrome c-releasing factor 21, CRF21, C7orf24 or LOC79017) is a 21 kDa AIG2-like family member. It is a cytosolic protein that induces the release of Cytochrome c from mitochondria, possibly initiating apoptosis. Human CRF21 is 188 amino acids (aa) in length and is a complex of beta -sheets and alpha -helices that create two terminal domains. It has been proposed that CRF21 forms homodimers. There are at least two possible splice variants of human CRF21, one showing a 70 aa substitution, while the second variant shows an 18 aa substitution for aa 97‑188. Full-length human CRF21 is 82% aa identical to mouse CRF21.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody (AF5086)(1)

Publication using AF5086

• Hama S, Arata M, Nakamura I Prevention of tumor growth by needle-free jet injection of anti-C7orf24 siRNA. Cancer Gene Ther., 2012-06-01;19(8):553-7. 2012-06-01 [PMID: 22653386] (Western Blot, Human)

Bioinformatics

• Gene Symbol: GGCT
• Uniprot:
  • O75223(Human)