Recombinant Human gamma-glutamyl hydrolase Protein

SDS-Page: Recombinant Human gamma-glutamyl hydrolase Protein [NBP1-78898]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human gamma-glutamyl hydrolase Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 25-318 of Human gamma-glutamyl hydrolase
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MRPHGDTAKK PIIGILMQKC RNKVMKNYGR YYIAASYVKY LESAGARVVP VRLDLTEKDY EILFKSINGI LFPGGSVDLR RSDYAKVAKI FYNLSIQSFD DGDYFPVWGT CLGFEELSLL ISGECLLTAT DTVDVAMPLN FTGGQLHSRM FQNFPTELLL SLAVEPLTAN FHKWSLSVKN FTMNEKLKKF FNVLTTNTDG KIEFISTMEG YKYPVYGVQW HPEKAPYEWK NLDGISHAPN AVKTAFYLAE FFVNEARKNN HHFKSESEEE KALIYQFSPI YTGNISSFQQ CYIFD

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: GGH
• Purity: >95% pure by SDS-PAGE

Applications/Dilutions

• Theoretical MW: 35.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol, 0.1 M NaCl
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >95% pure by SDS-PAGE

Alternate Names for Recombinant Human gamma-glutamyl hydrolase Protein

• Conjugase
• EC 3.4.19.9
• gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase)
• gamma-glutamyl hydrolase
• Gamma-Glu-X carboxypeptidase
• GH

Background

Gamma-glutamyl hydrolase, also known as GGH, is a homodimeric protein that catalyzes the cleavage of the gamma-glutamyl chain of folylpoly-gamma-glutamyl substrates and is a central enzyme in folyl and antifolyl poly-gamma-glutamate metabolism. GGH may play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates. Recombinant human GGH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for gamma-glutamyl hydrolase Recombinant Protein (NBP1-78898)(3)

Publications using NBP1-78898

• Zou Y, Duan H, Li L et al. Quantification of polyglutamyl 5-methyltetrahydrofolate, monoglutamyl folate vitamers, and total folates in different berries and berry juice by UHPLC-MS/MS Food Chemistry Mar 1 2019
• Zou Y, Duan H, Li L et al. Quantification of polyglutamyl 5-methyltetrahydrofolate, monoglutamyl folate vitamers, and total folates in different berries and berry juice by UHPLC-MS/MS bioRxiv Oct 13 2018 [PMID: 30409571]
• Stamm RA, Fazili Z, Pfeiffer CM. Addition of exogenous gamma-glutamyl hydrolase eliminates the need for lengthy incubation of whole blood lysate for quantitation of folate vitamers by high performance liquid chromatography-tandem mass spectrometry Current Developments inNutrition 2017 Nov 22 [PMID: 29955722] (Human)

Bioinformatics

• Gene Symbol: GGH