gamma Catenin [p Tyr550] Antibody

Immunocytochemistry/Immunofluorescence: gamma Catenin [p Tyr550] Antibody [NBP3-23198] - Immunocytochemical labeling of phosphorylated gamma Catenin in control (Top) and pervanadate-treated (Bottom) A431 cells. The cells were co-labeled with mouse monoclonal gamma Catenin (CM1111) or rabbit polyclonal gamma Catenin (Tyr-550) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy2 or Cy3.

Western Blot: gamma Catenin [p Tyr550] Antibody [NBP3-23198] - Western blot analysis of anti-gamma Catenin (C-terminal) immunoprecipitates from pervanadate-treated A431. The immunoprecipitates were untreated (lanes 1,3,7) or treated with alkaline phosphatase (lanes 2,4,8). The blots were probed with gamma Catenin (a.a. 545-555), gamma Catenin (Tyr-550) or gamma Catenin (C-terminal) antibodies. The anti-gamma Catenin (Tyr-550) was used in the presence of gamma Catenin (Tyr-550) (lane 5) or gamma Catenin (Tyr-644) (lane 6) peptides.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

gamma Catenin [p Tyr550] Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Phospho-gamma Catenin (Tyr-550) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 550 of human gamma Catenin. This peptide sequence is highly conserved in rat and mouse gamma Catenin. [UniProt# P14923]
• Modification: p Tyr550
• Specificity: This antibody was cross-adsorbed to both phospho-tyrosine coupled to agarose and to dephosphorylated gamma Catenin (Tyr-550) peptide before affinity purification using phospho-gamma Catenin (Tyr-550) peptide (without carrier). The antibody detects an 84kDa protein corresponding to the molecular mass of gamma Catenin on SDS-PAGE immunoblots of A431 and Hct116 src transformed cells treated with pervanadate (1 mM for 30 min), but not in control cells.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: JUP
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Western Blot 1:1000
• Theoretical MW: 84 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for gamma Catenin [p Tyr550] Antibody

• catenin (cadherin-associated protein), gamma (80kD)
• catenin (cadherin-associated protein), gamma 80kDa
• Catenin gamma
• Desmoplakin III
• desmoplakin-3
• DP3CTNNG
• DPIII
• gamma-catenin
• junction plakoglobin
• PDGBARVD12
• PKGB

Background

Catenin gamma, also known as Junction Plakoglobin, is a common junctional plaque protein. It is a cytoplasmic protein with soluble and membrane-associated forms. The presence of Catenin gamma in both the desmosomes and in the intermediate junctions suggests it has a role in the structure and Function of submembranous plaques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: JUP