Galectin-3 Antibody (M3/38) [Biotin]

Flow Cytometry: Galectin-3 Antibody (M3/38) [Biotin] [NBP1-43553] - Intracellular staining of the monocyte population in normal human peripheral blood cells (right) or C57BL/6 mouse bone marrow cells (left) with Anti-Mouse CD11b APC and 0.125 ug of Anti-Human/Mouse Galectin-3 Biotin followed by Streptavidin PE. Total viable cells were used for analysis.

• Reactivity: Mu
• Applications: Flow
• Clone: M3/38
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Biotin
• Concentration: 0.5 mg/ml

Galectin-3 Antibody (M3/38) [Biotin] Summary

• Immunogen: The immunogen for this antibody was Galectin3 Mac2.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: LGALS3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
• Application Notes: This M3/38 antibody has been tested by flow cytometric analysis of mouse PECs (thioglycollated elicied peritioneal cells). This can be used at less than or equal to 0.25 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS (pH 7.2) with 0.1% gelatin
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for Galectin-3 Antibody (M3/38) [Biotin]

• AGE-R3
• Carbohydrate-binding protein 35
• CBP 35,35 kDa lectin
• CBP35
• GAL3
• Gal-3
• galactin-3
• Galactose-specific lectin 3
• Galactoside-binding protein
• GALBPCBP35
• Galectin3
• Galectin-3
• GALIG
• IgE-binding protein
• L29
• L31
• L-31
• Laminin-binding protein
• Lectin L-29
• lectin, galactoside-binding, soluble, 3
• LGALS2
• LGALS3
• Mac-2 antigen
• Mac-2
• MAC2GAL3

Background

The M3/38 antibody reacts with mouse Galectin-3 (Mac-2). Galectins are a family of animal lectins which appear to exhibit a variety of biological functions. Galectin 3 (aka LGALS3, galactose-specific soluble lectin 3, Mac-2, L-29) is one of the more extensively studied members of this family and is a 30 kDa beta-galactoside-binding protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homo-oligomerized. Galectin 3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins. This monoclonal antibody M3/38 crossreacts to human Galectin-3 (LGALS3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LGALS3