GADD153/CHOP Antibody (S02-6A3)

Western Blot: GADD153/CHOP Antibody (S02-6A3) [NBP3-14934] - Western blot detection of GADD153/CHOP in K562, CHO-K1, Hela cell lysates using GADD153/CHOP Rabbit mAb (1:1000 diluted). Predicted band size: 19KDa. Observed band size: 27KDa.

Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (S02-6A3) [NBP3-14934] - Immunohistochemistry of GADD153/CHOP in paraffin-embedded Human breast cancer tissue using GADD153/CHOP Rabbit mAb at dilution 1/1

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: S02-6A3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

GADD153/CHOP Antibody (S02-6A3) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human GADD153/CHOP (Uniprot#: P35638)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DDIT3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1/20-1/50
  • Immunohistochemistry-Paraffin 1/20-1/50
  • Western Blot 1/1000-1/2000
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for GADD153/CHOP Antibody (S02-6A3)

• C/EBP-homologous protein 10
• C/EBP-homologous protein
• CCAAT/enhancer-binding protein homologous protein
• CEBP zeta
• CHOP
• CHOP10 CEBPZ
• CHOP10
• CHOP-10
• CHOPC/EBP zeta
• DDIT3
• DDIT-3
• DNA damage-inducible transcript 3 protein
• DNA-damage-inducible transcript 3
• GADD153
• Growth arrest and DNA damage-inducible protein GADD153

Background

GADD153 encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GADD153/CHOP Antibody (NBP3-14934)(1)

Publication using NBP3-14934

• Wang J, Jin J, Li G NR3C2 activates LCN2 transcription to promote endoplasmic reticulum stress and cell apoptosis in ischemic cerebral infarction Brain research 2023-10-11 [PMID: 37832761] (WB, Mouse)
  
  Details:
  1:500 dilution

Bioinformatics

• Gene Symbol: DDIT3