GAD1/GAD67 Antibody

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD1/GAD67 antibody (NBP3-48499) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD1/GAD67 antibody (NBP3-48499) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

GAD1/GAD67 antibody detects GAD1/GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.GAD1/GAD67 stained by GAD1/GAD67 antibody (NBP3-48499) diluted at 1:50.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GAD1/GAD67 antibody detects GAD1/GAD67 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: GAD1/GAD67 protein stained by GAD1/GAD67 antibody (NBP3-48499) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] diluted at 1:500.Blue: Fluoroshield with DAPI .

GAD1/GAD67 antibody detects GAD1/GAD67 protein expression by immunohistochemical analysis.Sample: Paraffin-Embedded adult mouse retina. Green: GAD1/GAD67 protein stained by GAD1/GAD67 antibody (NBP3-48499) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250.Blue: Fluoroshield with DAPI . ^[^GTX]br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD1/GAD67 antibody (NBP3-48499) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GAD1/GAD67 antibody detects GAD1/GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.GAD1/GAD67 stained by GAD1/GAD67 antibody (NBP3-48499) diluted at 1:50.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

GAD1/GAD67 Antibody Summary

• Description: Novus Biologicals Rabbit GAD1/GAD67 Antibody (NBP3-48499) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human GAD65/67. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GAD1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen Assay Dependent
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 67 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for GAD1/GAD67 Antibody

• 67kD)
• EC 4.1.1
• FLJ45882
• GAD1
• GAD25
• GAD67
• glutamate decarboxylase 1 (brain, 67kDa)
• Glutamate decarboxylase 67 kDa isoform

Background

GAD65/67 encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GAD1