GABPB1 Antibody - BSA Free

Immunohistochemistry-Paraffin: GABPB1 Antibody [NBP1-84028] - Staining of human appendix shows distinct nuclear positivity in glandular cells.

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Analysis in human cell line U-251 MG.

ChIP-Exo-Seq composite graph for Anti-GABPB1 (NBP1-84028) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GABPB1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit GABPB1 Antibody - BSA Free (NBP1-84028) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: QNQINTNPESPDTVTIHAATPQFIIGPGGVVNLTDETGVSAVQFGNSSTSVLATLAALAEASAPLSNSSETPVVATEEVVTAESVDGAIQQVVSS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GABPB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  GABPB1 Protein (NBP1-84028PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for GABPB1 Antibody - BSA Free

• E4TF1
• E4TF1-47
• E4TF1-53
• E4TF1BBABPB2
• GA binding protein transcription factor, beta subunit 1
• GA binding protein transcription factor, beta subunit 2
• GA-binding protein subunit beta-1
• GABP subunit beta-1
• GABPB-1
• GABPB2
• GABPB-2
• GABPBGABP subunit beta-2
• NRF2B1
• NRF2B2
• Nuclear respiratory factor 2
• Transcription factor E4TF1-47
• Transcription factor E4TF1-53

Background

GABPB1 encodes the GA-binding protein transcription factor, beta subunit. This protein forms a tetrameric complex with the alpha subunit, and stimulates transcription of target genes. The encoded protein may be involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. The crystal structure of a similar protein in mouse has been resolved as a ternary protein complex. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GABPB1