Western Blot: GABPA Antibody (M1) [H00002551-M03] - Using 15 ug of human glioblastoma stem cells lysates. Primary antibody: GABPA dilution at 1:500 in 2.5% BSA/TBST. This image was submitted via customer Review.
Immunocytochemistry/ Immunofluorescence: GABPA Antibody (M1) [H00002551-M03] - Analysis of monoclonal antibody to GABPA on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: GABPA Antibody (M1) [H00002551-M03] - Analysis of GABPA expression in Hela S3 NE (Cat # L013V3).
Western Blot: GABPA Antibody (M1) [H00002551-M03] - Analysis of GABPA expression in transfected 293T cell line by GABPA monoclonal antibody (M03), clone M1. Lane 1: GABPA transfected lysate (Predicted MW: 51.3 KDa). ...read more
ELISA: GABPA Antibody (M1) [H00002551-M03] - Detection limit for recombinant GST tagged GABPA is approximately 1ng/ml as a capture antibody.
Immunoprecipitation: GABPA Antibody (M1) [H00002551-M03] - Analysis of GABPA transfected lysate using anti-GABPA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GABPA monoclonal antibody.
GABPA (AAH35031, 1 a.a. ~ 454 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTKREAEELIEIEIDGTEKAECTEESIVEQTYAPAECVSQAIDINEPIGNLKKLLEPRLQCSLDAHEICLQDIQLDPERSLFDQGVKTDGTVQLSVQVISYQGIEPKLNILEIVKPADTVEVVIDPDAHHAESEAHLVEEAQVITLDGTKHITTISDETSEQVTRWAAALEGYRKEQERLGIPYDPIQWSTDQVLHWVVWVMKEFSMTDIDLTTLNISGRELCSLNQEDFFQRVPRGEILWSHLELLRKYVLASQEQQMNEIVTIDQPVQIIPASVQSATPTTIKVINSSAKAAKVQRAPRISGEDRSSPGNRTGNNGQIQLWQFLLELLTDKDARDCISWVGDEGEFKLNQPELVAQKWGQRKNKPTMNYEKLSRALRYYYDGDMICKVQGKRFVYKFVCDLKTLIGYSAAELNRLVTECEQKKLAKMQLHGIAQPVTAVALSTASLQTEKDN
GABPA - GA binding protein transcription factor, alpha subunit 60kDa
Test in a species/application not listed above to receive a full credit towards a future purchase.
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
In 1x PBS, pH 7.4
Quality control test: Antibody Reactive Against Recombinant Protein.
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for GABPA Antibody (M1)
E4TF1ATranscription factor E4TF1-60
GA binding protein transcription factor, alpha subunit 60kDa
GA-binding protein alpha chain
GA-binding protein transcription factor, alpha subunit (60kD)
GABP subunit alpha
human nuclear respiratory factor-2 subunit alpha, 10
nuclear respiratory factor 2 alpha subunit
Nuclear respiratory factor 2 subunit alpha
This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
SDS-PAGE in 10% polyacrylamide gel at constant 110 volts for 120 min. Blotting onto PVDF membrane at constant 300 mAmp 90 min at 4C. Primary antibody: GABPA dilution at 1:500 in 2.5% BSA/TBST, incubate at 4C overnight. Secondary antibody: anti-mouse IgG HRP conjugated dilution at 1:5000 in 2.5% BSA/TBST, incubate at RT for 1 hour.
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.