GABPA Antibody (M1)

Images

 
Western Blot: GABPA Antibody (M1) [H00002551-M03] - Using 15 ug of human glioblastoma stem cells lysates. Primary antibody: GABPA dilution at 1:500 in 2.5% BSA/TBST. This image was submitted via customer Review.
Immunocytochemistry/ Immunofluorescence: GABPA Antibody (M1) [H00002551-M03] - Analysis of monoclonal antibody to GABPA on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: GABPA Antibody (M1) [H00002551-M03] - Analysis of GABPA expression in Hela S3 NE (Cat # L013V3).
Western Blot: GABPA Antibody (M1) [H00002551-M03] - Analysis of GABPA expression in transfected 293T cell line by GABPA monoclonal antibody (M03), clone M1. Lane 1: GABPA transfected lysate (Predicted MW: 51.3 KDa). ...read more
ELISA: GABPA Antibody (M1) [H00002551-M03] - Detection limit for recombinant GST tagged GABPA is approximately 1ng/ml as a capture antibody.
Immunoprecipitation: GABPA Antibody (M1) [H00002551-M03] - Analysis of GABPA transfected lysate using anti-GABPA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GABPA monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clone
M1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

GABPA Antibody (M1) Summary

Immunogen
GABPA (AAH35031, 1 a.a. - 454 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTKREAEELIEIEIDGTEKAECTEESIVEQTYAPAECVSQAIDINEPIGNLKKLLEPRLQCSLDAHEICLQDIQLDPERSLFDQGVKTDGTVQLSVQVISYQGIEPKLNILEIVKPADTVEVVIDPDAHHAESEAHLVEEAQVITLDGTKHITTISDETSEQVTRWAAALEGYRKEQERLGIPYDPIQWSTDQVLHWVVWVMKEFSMTDIDLTTLNISGRELCSLNQEDFFQRVPRGEILWSHLELLRKYVLASQEQQMNEIVTIDQPVQIIPASVQSATPTTIKVINSSAKAAKVQRAPRISGEDRSSPGNRTGNNGQIQLWQFLLELLTDKDARDCISWVGDEGEFKLNQPELVAQKWGQRKNKPTMNYEKLSRALRYYYDGDMICKVQGKRFVYKFVCDLKTLIGYSAAELNRLVTECEQKKLAKMQLHGIAQPVTAVALSTASLQTEKDN
Specificity
GABPA - GA binding protein transcription factor, alpha subunit 60kDa
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GABPA
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.
Reviewed Applications
Read 1 Review rated 3
using
H00002551-M03 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GABPA Antibody (M1)

  • E4TF1-60
  • E4TF1ATranscription factor E4TF1-60
  • GA binding protein transcription factor, alpha subunit 60kDa
  • GA-binding protein alpha chain
  • GA-binding protein transcription factor, alpha subunit (60kD)
  • GABP subunit alpha
  • human nuclear respiratory factor-2 subunit alpha, 10
  • NFT2
  • NRF2
  • NRF2A
  • nuclear respiratory factor 2 alpha subunit
  • Nuclear respiratory factor 2 subunit alpha

Background

This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for GABPA Antibody (H00002551-M03) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00002551-M03:
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Images Ratings Applications Species Date Details
Western Blot GABPA H00002551-M03
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reviewed by:
Tanwarat Sanvoranart
WB Human 05/02/2017
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Summary

ApplicationWestern Blot
Sample Testedhuman glioblastoma
SpeciesHuman
Lot06307-S51

Comments

CommentsSDS-PAGE in 10% polyacrylamide gel at constant 110 volts for 120 min. Blotting onto PVDF membrane at constant 300 mAmp 90 min at 4C. Primary antibody: GABPA dilution at 1:500 in 2.5% BSA/TBST, incubate at 4C overnight. Secondary antibody: anti-mouse IgG HRP conjugated dilution at 1:5000 in 2.5% BSA/TBST, incubate at RT for 1 hour.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GABPA Antibody (H00002551-M03) (0)

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Secondary Antibodies

 

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The effects of ethanol consumption on glutamate production and xCT
xCT is a sodium independent glutamate transporter that regulates the exchange of extracellular l-cystine and intracellular l-glutamate across the plasma membrane. This process is critical to glutathione production and protection from subsequent ox...  Read full blog post.

The effects of ethanol consumption on glutamate production and xCT
xCT is a sodium independent glutamate transporter that regulates the exchange of extracellular l-cystine and intracellular l-glutamate across the plasma membrane. This process is critical to glutathione production and protection from subsequent ox...  Read full blog post.

The effects of ethanol consumption on glutamate production and xCT
xCT is a sodium independent glutamate transporter that regulates the exchange of extracellular l-cystine and intracellular l-glutamate across the plasma membrane. This process is critical to glutathione production and protection from subsequent ox...  Read full blog post.

The effects of ethanol consumption on glutamate production and xCT
xCT is a sodium independent glutamate transporter that regulates the exchange of extracellular l-cystine and intracellular l-glutamate across the plasma membrane. This process is critical to glutathione production and protection from subsequent ox...  Read full blog post.

The effects of ethanol consumption on glutamate production and xCT
xCT is a sodium independent glutamate transporter that regulates the exchange of extracellular l-cystine and intracellular l-glutamate across the plasma membrane. This process is critical to glutathione production and protection from subsequent ox...  Read full blog post.

The effects of ethanol consumption on glutamate production and xCT
xCT is a sodium independent glutamate transporter that regulates the exchange of extracellular l-cystine and intracellular l-glutamate across the plasma membrane. This process is critical to glutathione production and protection from subsequent ox...  Read full blog post.

The effects of ethanol consumption on glutamate production and xCT
xCT is a sodium independent glutamate transporter that regulates the exchange of extracellular l-cystine and intracellular l-glutamate across the plasma membrane. This process is critical to glutathione production and protection from subsequent ox...  Read full blog post.

The effects of ethanol consumption on glutamate production and xCT
xCT is a sodium independent glutamate transporter that regulates the exchange of extracellular l-cystine and intracellular l-glutamate across the plasma membrane. This process is critical to glutathione production and protection from subsequent ox...  Read full blog post.

The effects of ethanol consumption on glutamate production and xCT
xCT is a sodium independent glutamate transporter that regulates the exchange of extracellular l-cystine and intracellular l-glutamate across the plasma membrane. This process is critical to glutathione production and protection from subsequent ox...  Read full blog post.

The effects of ethanol consumption on glutamate production and xCT
xCT is a sodium independent glutamate transporter that regulates the exchange of extracellular l-cystine and intracellular l-glutamate across the plasma membrane. This process is critical to glutathione production and protection from subsequent ox...  Read full blog post.

Showing 1-10 of 49 blog posts - Show all blog posts.

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Tanwarat Sanvoranart
05/02/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol GABPA
Entrez
OMIM
Uniprot